[Histonet] Microwave tissue processing [Scanned By SOPHOS
Anti-Virus]
G.A.McHardy <@t> arh.grampian.scot.nhs.uk
G.A.McHardy <@t> arh.grampian.scot.nhs.uk
Wed Jun 2 08:56:02 CDT 2004
I would be interseted to hear from any lab with experience of rapid
microwave processing of small biopsies for urgent diagnosis. We did have a
machine on demo for a short time and now we are considering the pros and
cons before deciding whether or not to purchase.
Thank you for any help
Graham A McHardy
Pathology dept
Aberdeen Royal infirmary
01224 553852
> -----Original Message-----
> From: histonet-request <@t> lists.utsouthwestern.edu
> [SMTP:histonet-request <@t> lists.utsouthwestern.edu]
> Sent: 01 June 2004 18:03
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 7, Issue 1 [Scanned By SOPHOS
> Anti-Virus]
>
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> Today's Topics:
>
> 1. Rabbit anti-FITC-HRP conjugate (Yin Ping Lee)
> 2. VEGF problems in Immunohistochemistry (Miguel Valenzuela)
> 3. apoptosis protocol (anexin, caspase) for immunohistochemistry
> (Miguel Valenzuela)
> 4. Older embedding center in ebay. (HCS)
> 5. methyl green counterSTAiNing (Inga Hansson)
> 6. IHC_swine vessels_cross reactivity (Melanie Black)
> 7. RE: IHC_swine vessels_cross reactivity (Flynn, Evelyn)
> 8. RE: caspase 3 and pcna (C.M. van der Loos)
> 9. TRAP staining (Myri37 <@t> aol.com)
> 10. RE: Histonet Digest, Vol 6, Issue 9 (Shawn Salesky)
> 11. Re: IHC_swine vessels_cross reactivity (Gayle Callis)
> 12. High resolution with pole piece... (Nejat Y?lmaz)
> 13. Steve Slap (Traczyk7 <@t> aol.com)
> 14. Info required on antibodies (carmen loiselle)
> 15. Oddball formalin question! (Greg Dobbin)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 31 May 2004 11:57:40 -0700
> From: Yin Ping Lee <ylee <@t> bccancer.bc.ca>
> Subject: [Histonet] Rabbit anti-FITC-HRP conjugate
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <6BAF4D075F07D411B30900508B94CBA0C1F564 <@t> SERVER20>
> Content-Type: text/plain
>
> Hi, Histonetter:
>
> Does anyone have experience with rabbit anti-FITC-HRP conjugate from Dako?
> Does it work not as well and what is the problem exactly? I am using this
> product for the EBER ISH staining and have occasionally encountered
> mysterious background staining.
>
> Yin Ping Lee
> Histopathology Lab
> BC Cancer Agency
> Vancouver, BC
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 31 May 2004 23:09:55 +0200 (CEST)
> From: Miguel Valenzuela <mivalmar <@t> yahoo.es>
> Subject: [Histonet] VEGF problems in Immunohistochemistry
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20040531210955.52279.qmail <@t> web12609.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi all
>
> I need to find a paper to know what kind of cells are
> stained with VEGF (Vascular endotelial growth
> factor)...
> Yes, I know that endothelial cells are stained but I
> get also stain in adipose cells.
> I want to learn more about this antibody and others
> (tie, angiostatin, VEGF-Receptors 1,2)
>
> Any help will be useful.
> Thanks very much.
>
> Miguel Valenzuela.
> Spain.
>
> __________________________________________________
> Correo Yahoo! - 6 MB, antivirus y antispam ¡gratis!
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>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 31 May 2004 23:12:02 +0200 (CEST)
> From: Miguel Valenzuela <mivalmar <@t> yahoo.es>
> Subject: [Histonet] apoptosis protocol (anexin, caspase) for
> immunohistochemistry
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20040531211202.87675.qmail <@t> web12607.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi all
> I would like to contact someone that has used anexin-V
> and/or caspase-3 in paraffin tissues.
> I have a lot of nuclear stain with tunel.
> And I would like to find a protocol.
>
> Any help will be useful
> Thanks.
> Miguel V-S.
> Spain.
>
>
>
>
> ______________________________________________________________________
> Correo Yahoo! - 6MB, más protección contra el spam ¡Gratis!
> http://correo.yahoo.es
>
>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 31 May 2004 16:56:02 -0700
> From: "HCS" <int09018 <@t> alphahunt.com>
> Subject: [Histonet] Older embedding center in ebay.
> To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000b01c4476a$d55f13e0$6501a8c0 <@t> hp>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi, I have listed a spare embedding center that I no longer need on ebay
> in case anyone would be interested in getting one. The item number is
> 3818030988. It is a Reichert-Jung.
>
>
> LeRoy Brown HT(ASCP) HTL
>
> Histology Consultation Services
> 1-360-966-7300
> www.histocs.com
>
> Everson, Washington.
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 1 Jun 2004 07:51:56 +0200
> From: Inga Hansson <Inga.Hansson <@t> neuro.uu.se>
> Subject: [Histonet] methyl green counterSTAiNing
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <a05200f04bce1c8e75048@[130.238.32.31]>
> Content-Type: text/plain; charset="us-ascii" ; format="flowed"
>
> Thank you Sharon C......for the advice!!!!!!
> Maybe I just go and make a new one.......!
> How long do you keep sections in the solution??Is five minutes too
> little??
>
> Regards
>
> Inga
>
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 1 Jun 2004 10:09:33 +0200
> From: Melanie Black <ctsblack <@t> capeheart.uct.ac.za>
> Subject: [Histonet] IHC_swine vessels_cross reactivity
> To: histonet <@t> pathology.swmed.edu
> Message-ID: <a05100304bce1e920c9f5@[196.21.12.32]>
> Content-Type: text/plain; charset="us-ascii" ; format="flowed"
>
>
> The Abcam mavrophage marker (Ab 8740) and the Dako Mac 387(M0747)
> claim to cross react with swine. The resin sections would need to de
> resin the sections first.
>
> Melanie.
>
> Dear all,
>
> I need to identify macrophages and endothelial cells in resin embedded
> pig blood vessels with stent. I couldnt find any specific primary
> antibodies against swine macrophages or endothelial cells and has to
> rely on cross reactivity pirmarily intended in human tissue. Has any one
> tried such cross reactive antibodies in swine tissue or is there
> specific ones against pig which ive missed. Please help
>
> J. Raghul, Scientist -C, sri chithra tirunal institute of medical
> sciences, Trivandrum , India
>
> --
> Cardiovascular Research Unit
> Div. of Cardiothoracic Surgery
> Chris Barnard Building
> University of Cape Town
> Anzio Road
> Observatory
> 7925
> Republic of South Africa
>
> Tel +27 21 406-6589
> Cel +27 82 469-3352
> Fax +27 21 448-5935
>
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 1 Jun 2004 09:56:20 -0400
> From: "Flynn, Evelyn" <Evelyn.Flynn <@t> childrens.harvard.edu>
> Subject: RE: [Histonet] IHC_swine vessels_cross reactivity
> To: "Dr. Raghul" <raghul <@t> sctimst.ker.nic.in>,
> histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <A75ECE3D2DE43D4CB5A9F3B55177D8CA0F469B <@t> CHEXV2.CHBOSTON.ORG>
> Content-Type: text/plain; charset=iso-8859-1
>
> Dear Dr. Raghul,
> The rabbit polyclonal antibody against von Willebrand factor (Factor
> VIII-related antigen;
> DAKO Cat. # A0082) cross-reacts with most species except rabbit. I have
> tested it with paraffin
> sections but not with resin, however.
>
> Sincerely,
> Evelyn Flynn
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Dr. Raghul
> Sent: Mon 5/31/2004 12:09 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] IHC_swine vessels_cross reactivity
>
> Dear all,
>
> I need to identify macrophages and endothelial cells in resin embedded
> pig blood vessels with stent. I couldnt find any specific primary
> antibodies against swine macrophages or endothelial cells and has to
> rely on cross reactivity pirmarily intended in human tissue. Has any one
> tried such cross reactive antibodies in swine tissue or is there
> specific ones against pig which ive missed. Please help
>
> J. Raghul, Scientist -C, sri chithra tirunal institute of medical
> sciences, Trivandrum , India
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Tue, 01 Jun 2004 16:16:37 +0200
> From: "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
> Subject: [Histonet] RE: caspase 3 and pcna
> To: histonet <@t> lists.utsouthwestern.edu
> Cc: pruegg <@t> colobio.com
> Message-ID: <29f0af29f78d.29f78d29f0af <@t> amc.uva.nl>
> Content-Type: text/plain; charset=us-ascii
>
> Dear Patsy,
> We used a anti-caspase-3 rabbit antibody from Cell Signaling (#9661) on
> FFPE sections in a 1/200 dilution (60 min, RT) after HIER with Tris/EDTA
> pH9.0. This means that the epitope survives both formalin-fixation and
> embedding through alcohols. In your situation you may to fix the cells at
> the chamber slide with 4% PFA (or routinely buffered formalin) for 5 min
> and perform your caspase-3 staining. As it is nuclear staining perhaps you
> need to add 0.1% saponin to all antibody steps and washing buffers to open
> up the cell membranes letting your antibodies go in and out.
> With respect to PCNA (we call it a poorman Ki67!!) you may try to replace
> it by anti-Ki67, rabbit monoclonal SP-6 (Neomarkers). In our hands it
> reacts with human, mouse and rat proliferating nuclei on FFPE's and cryo's
> without any non-specific background staining.
> Hope this helps!
> Chris van der Loos, PhD
> Dept. of Pathology
> Academic Medical Center
> Amsterdam - The Netherlands
>
> ---- Original Message -----
> >From Patsy Ruegg <pruegg <@t> colobio.com>
> Date Thu, 27 May 2004 13:09:18 -0600
> To Jackie.O'Connor <@t> abbott.com
> Cc histonet <@t> lists.utsouthwestern.edu
> Subject [Histonet] caspase 3 and pcna
> Jackie and all, could you please advise me on doing caspase 3 and pcna on
> cells grown on chamber slides, I mostly need advise with caspase, what
> antibody do you use?
> Thank you all,
> Patsy
>
>
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Tue, 01 Jun 2004 10:11:08 -0400
> From: Myri37 <@t> aol.com
> Subject: [Histonet] TRAP staining
> To: histonet <@t> pathology.swmed.edu
> Message-ID: <032BBAD4.24B06E2E.0005167B <@t> aol.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi
> I would like to try SIGMA kit N°85, for staining osteoclats on fresh fixed
> cells (reconstructed tissue, and also paraffin embedded secions. Tissue is
> fixed in 4 % paraformaldehyde, is there another fixative wich works better
> ?
> Do you think if i must change the way to proceede ?
>
> Thank you very much
> Myriam baali
> Natural implant
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Tue, 1 Jun 2004 10:54:40 -0400
> From: Shawn Salesky <ssalesky <@t> LowellGeneral.org>
> Subject: [Histonet] RE: Histonet Digest, Vol 6, Issue 9
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>, Shawn Salesky
> <ssalesky <@t> LowellGeneral.org>
> Message-ID: <9E49B4E3A6200C4B91E5FB99FB1F7EDB571F37 <@t> NTMAIL>
> Content-Type: text/plain
>
>
> Hi All,
>
> I am interested in finding out which CPT code people are using for
> reimbursment of INFORM HPV testing on the Ventana system, from Cytyc
> thin-prep slides.
>
> Any insight would be helpful.
>
>
>
>
> This e-mail and any attachments is intended only for the person or
> entity to which it is addressed and may contain confidential and
> privileged information. If you are not the intended recipient,
> please notify the sender immediately by return e-mail, delete this
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> information by a person other than the intended recipient is
> unauthorized and may be illegal.
>
>
>
> ------------------------------
>
> Message: 11
> Date: Tue, 01 Jun 2004 08:59:31 -0600
> From: Gayle Callis <gcallis <@t> montana.edu>
> Subject: Re: [Histonet] IHC_swine vessels_cross reactivity
> To: "Dr. Raghul" <raghul <@t> sctimst.ker.nic.in>,
> Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <3.0.6.32.20040601085931.00c17958 <@t> gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> You can try to find the antibodies at SEROTEC, they can tell you what
> cross
> reacts to swine from their cross reactivity chart. This may be on their
> website. If the resin is Glycol methacrylate plastic, you will will have
> poor success with immunostaining. IF the tissue is embedded in
> methylmethacrylate, you can use Neil Hands methods for retrieval, and
> remove the plastic COMPLETELY. This has been discussed on Histonet
> before,
> you can do a search in Histonet archives.
>
>
>
> At 09:39 AM 5/31/2004 +0530, you wrote:
> >Dear all,
> >
> >I need to identify macrophages and endothelial cells in resin embedded
> >pig blood vessels with stent. I couldnt find any specific primary
> >antibodies against swine macrophages or endothelial cells and has to
> >rely on cross reactivity pirmarily intended in human tissue. Has any one
> >tried such cross reactive antibodies in swine tissue or is there
> >specific ones against pig which ive missed. Please help
> >
> > J. Raghul, Scientist -C, sri chithra tirunal institute of medical
> > sciences, Trivandrum , India
> >
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
>
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Tue, 1 Jun 2004 18:10:15 +0300
> From: Nejat Y?lmaz <nyilmaz <@t> mersin.edu.tr>
> Subject: [Histonet] High resolution with pole piece...
> To: "histonet" <histonet <@t> pathology.swmed.edu>,
> <ListServer <@t> Microscopy.com>
> Message-ID: <000801c447ea$8b8b5460$4e01a8c0 <@t> mersin.edu.tr>
> Content-Type: text/plain; charset="windows-1254"
>
> Hello Again...
>
> We are wondering resolution of a TEM (i.e. JEM1011) can be increased
> effectively with a part called pole piece? Does it really work?
> Thanks in advance...
>
> Dr. Necat Yilmaz
>
> ------------------------------
>
> Message: 13
> Date: Tue, 1 Jun 2004 11:05:46 EDT
> From: Traczyk7 <@t> aol.com
> Subject: [Histonet] Steve Slap
> To: HistoNet <@t> pathology.swmed.edu
> Message-ID: <11a.32fcd3cb.2dedf54a <@t> aol.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> This message is being posted for Lisa:
>
> Hello Everyone,
> All of your well wishes and prayers are very much appreciated by Jeremy
> and
> myself. Steven sustained serious injury in the accident. He seems to be
> healing well from most. The most serious is the head trauma, which I
> totally
> believe he will awaken from (probably asking where the best restaurant
> is). Please keep praying and sending good vibes. I will let you know when
> there is any change. I have been speaking with Dorothy and she offered to
> be
> the contact person. Please contact her for updates. Anything that you
> would
> like to send via email, Dorothy can forward to us.
>
> Peace and many thanks to you all, Lisa
>
> Note from Dorothy:
> Keep those cards and letters coming in. A reminder, the regular mail
> address
> is:
> Lisa Smith & Steve Slap
> PO Box 31
> Lake Pleasant, MA 01347
>
>
> ------------------------------
>
> Message: 14
> Date: Tue, 01 Jun 2004 11:46:37 -0400
> From: "carmen loiselle" <carmen_loiselle <@t> hotmail.com>
> Subject: [Histonet] Info required on antibodies
> To: histonet <@t> pathology.swmed.edu
> Message-ID: <BAY19-F355TetpIuPHD0001ca9a <@t> hotmail.com>
> Content-Type: text/plain; charset=iso-8859-1; format=flowed
>
> Hello everyone,
>
> Does anyone have any information regarding the following antibodies;
> P19(INK4d), Kip1/P27 and fli-1 ??? These antibodies would be used on
> paraffin sections for immunohistochemistry . We also have Ventana
> immunostainer (NEXES and Benchmark) .
>
>
> Any input would be greatly appreciated . Thank you in advance.
>
> Carmen
>
> _________________________________________________________________
> MSN Messenger : discutez en direct avec vos amis !
> http://messenger.fr.msn.ca/
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Tue, 1 Jun 2004 13:54:59 ADT
> From: "Greg Dobbin" <dobbin <@t> upei.ca>
> Subject: [Histonet] Oddball formalin question!
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <40BC8AB2.2212.1174A4C <@t> localhost>
> Content-Type: text/plain; charset=US-ASCII
>
> Hello All,
> Here is this weeks oddball question! I am tring to make latex casts
> of air sacs in birds (the veterinary people will know that birds have
> air sacs and pneumatic bones that work in conjuction with the lungs
> to facilitate O2 uptake [among other functions such as temp. reg.]).
>
> First of all, we will gently compress the birds body (these birds are
> found dead and brought in by the public-we are not euthanizing!) to
> force out excess air. Then, while suspending the bird by the head
> we will run liquid latex into the trachea. We hope to make casts of
> the communications between the lungs and the various air sacs
> and subcutaneous air pockets. Some these diverticuli are quite
> small so we think we will have to dilute the latex to make it thin
> enough to pass thru these tiny openings.
>
> So since we are diluting the latex anyway, why not dilute it with
> formalin (10% NBFS) so that we simultaneously begin to fix the
> surrounding structures? However, diluting the latex means that, by
> default, the formalin is also being diluted. My question then is this:
> "What would happen if I made the formalin up partly with latex so
> that the final concentration of formalin in my latex-formalin mixture,
> would be 10% and the latex concentration would still be high
> enough to effectively polymerize?"
>
> (I have already tried diluting latex with water and it still polymerized
> at a dilution of 1:4 following 18 hours at -20 C [checked after
> thawing of course]).
>
> I'm thinking the osmotic pressures would be out of whack and the
> formalin probably won't penetrate very far from the latex cast
> anyway, but I thought some you with better knowledge of formalin
> fixation could offer some insight or suggestions before I start
> playing around with this.
>
> I can't wait see the replies on this one!!
> Cheers!
> Greg
>
>
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 University Ave.
> Charlottetown, P.E.I.
> Canada, C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Making a living is getting; making a life is giving.
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 7, Issue 1
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