[Histonet] Ki-67 (MIB1) Immunohistochemistry and neutrophil labeling

Wed Jun 2 09:47:52 CDT 2004

It's unlikely to be endogenous peroxidase.  I compared 3% hydrogen 
peroxide(aq) - 10min with 3% hydrogen peroxide in methanol - 30min with 
the same results; negative isotype control, positive neutrophils in the 
DAB test section.  Note: Dako Biotin Blocking system was also used in the 
protocol.

David L. Krull
Scientist, Molecular & 
Ultrastructural Pathology
GSK Safety Assessment 9.2103
5 Moore Drive
RTP, NC 27709-3398

Phone: 919-315-2457
David.L.Krull <@t> GSK.com

"Edwards, R.E." <ree3 <@t> leicester.ac.uk>

02-Jun-2004 09:59

        To:     david.l.krull <@t> gsk.com

        cc: 
        Subject:        RE: [Histonet] Ki-67 (MIB1) Immunohistochemistry and neutrophil labeling

Endogenous peroxidase???????????????

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
david.l.krull <@t> gsk.com
Sent: 02 June 2004 14:50
To: histonet <@t> lists.utsouthwestern.edu
Cc: charles.w.qualls <@t> gsk.com
Subject: [Histonet] Ki-67 (MIB1) Immunohistochemistry and neutrophil
labeling

We recently had a Cyno monkey study which required evaluation of cell 
proliferation in formalin fixed, paraffin embedded liver sections.  During 

the review of the slides we noticed that neutrophils were also positive in 

addition to proliferating hepatocytes using DAB as the chromagen.  When I 
switched to Vector VIP the neutrophils were negative.  Has anyone else 
observed neutrophil labeling with Ki-67 using  DAB?  Could the DAB be 
reacting with the neutrophils?

David L. Krull
Scientist, Molecular & 
Ultrastructural Pathology
GSK Safety Assessment 9.2103
5 Moore Drive
RTP, NC 27709-3398

Phone: 919-315-2457
David.L.Krull <@t> GSK.com

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