[Histonet] Formalin fix---then freeze?

[Greer, Patricia]

Kathy,

Yes, we have frozen formalin fixed tissues in several instances in the
past.  We would rinse the formalin (or 70% alcohol if that is what the
tissue is stored in) and place the tissue in an embedding mold filled
with OCT and freeze onto a chuck.  This worked well for a number of
tissues and was helpful in doing a quick special stain or
immunohistochemistry.


Pat Greer
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road
Atlanta, GA 30333


 Is it possible to formalin fix a tissue and then put it in OCT and
freeze it to cut frozen sections?  

Kathy Mink