[Histonet] Formalin fix---then freeze?

[GUTIERREZ, JUAN]

Yes!  I used to get this request quite often.  Just trim the tissue you're going to freeze, put it in a cassette and wash it under running deionized water for a couple of hours.  Pat it dry and embed in OCT.  I've done it with heart, lung and liver and it works very well.  Good luck.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
Christus Santa Rosa Hospital
333 N. Santa Rosa Ave.
San Antonio, TX  78207
(210)704-2533


-----Original Message-----
From: KMIN (Kathy Mink) [mailto:minkk <@t> zgi.com]
Sent: Monday, July 12, 2004 7:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formalin fix---then freeze?


Hi,

I have been asked an unusual (stupid?) question and hope someone can help.  Is it possible to formalin fix a tissue and then put it in OCT and freeze it to cut frozen sections?  I know it doesn't make much sense but apparently a contract we have was worded this way (obviously not proof read by the pathologist) and now I need to treat the tissue this way if possible.  Help!

Kathy Mink


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