[Histonet] Re: Dezenkerize
GUTIERREZ, JUAN
juan.gutierrez <@t> christushealth.org
Tue Jul 13 09:02:45 CDT 2004
Just curious. How do you mixed the iodine with the xylene?
Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
Christus Santa Rosa Hospital
333 N. Santa Rosa Ave.
San Antonio, TX 78207
(210)704-2533
-----Original Message-----
From: Karen Wittler [mailto:kwittle <@t> jhmi.edu]
Sent: Tuesday, July 13, 2004 8:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Dezenkerize
To remove Mercury precipitate pigments we use iodine in xylene when the
slides come out of the oven, then deparaffinize and stain as usual. I
imagine this would work with an automated stainer.
Karen Wittler, HT(ASCP)
Johns Hopkins Hospital
Baltimore, MD
>>> histonet-request <@t> lists.utsouthwestern.edu 07/10/04 01:05PM >>>
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Today's Topics:
1. Re: Sakura Tissue Processor [Histonet Digest, Vol 8, Issue
15] (tony.j.savage <@t> gsk.com)
2. (no subject) (Achstetter, Virginia A.)
3. Mohs Histotech; Xylene substitute (Michael Todd)
4. DEZENKERIZE USING SAKURA DRS STAINER (Shelkowsky, Ben)
5. Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER
(Fred Underwood)
6. Re: (no subject) (SMITH,REBEKAH FELICIA)
7. re: 266mp manual (Pereira, Laurie )
8. RE: B-Plus substitute for B5 (Moklebust, Amanda C)
9. unsubscribe (ANN MARUSKA)
10. RE: B-Plus substitute for B5 (Wiese, Jason VHAROS)
11. RE: B-Plus substitute for B5 (Luck, Greg D.)
12. Re: Room Temperature (Muhammad Tahseen)
13. Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER
(lpwenk <@t> sbcglobal.net)
14. Re: Room Temperature (lpwenk <@t> sbcglobal.net)
15. PROSTATE BIOSIES (Winters, Bert)
----------------------------------------------------------------------
Message: 1
Date: Fri, 9 Jul 2004 18:41:44 +0100
From: tony.j.savage <@t> gsk.com
Subject: [Histonet] Re: Sakura Tissue Processor [Histonet Digest, Vol
8, Issue 15]
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <OF3D956CBF.DD6664BD-ON80256ECC.0060D4A5 <@t> sb.com>
Content-Type: text/plain; charset=us-ascii
From: Juile Gorenstein <j_gorenstein <@t> yahoo.com> wrote
"Hello all,
I was wondering if anyone has had problems with xylene fumes using
Sakura
VIP Tissue processor. Ours is currently sitting on a bench in a room
with
a snorkle right above is and the smell is still really bad and is
causing
headaches for people who try to work in that room. Does anyone have
any
suggestions?
Thank you,
Julie
Novartis Institues for Biomedical Research
Cambridge, MA"
It sounds as though it is time to replace the carbon filter on
the
VIP processor. This should be done on a regular basis depending on
usage.
Regards,
Tony
Histopathology Group
Asthma Biology Department.
RIRP CEDD.
GlaxoSmithKline Medicines Research Centre,
Gunnelswood Road,
STEVENAGE,
Hertfordshire.
SG1 2NY
tel. +44 (0)1438 764117
fax. +44 (0)1438 764782
email. Tony.J.Savage <@t> gsk.com
mobile +44 07753609835
http://ukdiscovery.gsk.com/histopathology/default.htm
------------------------------
Message: 2
Date: Fri, 9 Jul 2004 14:53:10 -0400
From: "Achstetter, Virginia A." <ACHSTETT <@t> afip.osd.mil>
Subject: [Histonet] (no subject)
To: "Histonet \(E-mail\)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9C631520464F9E4BA5B111450F2A0AAD0BC9FB <@t> lewis.afip.osd.mil>
Content-Type: text/plain; charset="iso-8859-1"
Our department wants to purchase a tissue arrayer from Beecher
Instruments. All attempts to contact them has failed. Email address is
no longer valid. Fax goes unanswered as well as phone and snail mail.
Does anyone know where we could purchase this equipment or how to get in
touch with Beecher Instruments?
Ginny Achstetter HT (ASCP)
Armed Forces Institute of Pathology
Soft Tissue Pathology
6825 16th St. NW
Bldg 54 Rm. 3118
Washington, DC 20306
202-782-2813
------------------------------
Message: 3
Date: Fri, 9 Jul 2004 15:09:24 -0400
From: "Michael Todd" <mtodd <@t> medscape.com>
Subject: [Histonet] Mohs Histotech; Xylene substitute
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <C4B0B656DE4B70E42BEBE9F2411686BF <@t> mtodd.medscape.com>
Content-Type: text/plain; charset=iso-8859-1
I am opening a Mohs laboratory in Northern Virginia, just outside
Washington DC, and need some help.
1. I need a Histotech, either PT or FT. Beginning September, 2004.
2. I have been following the conversation about xylene substitutes -
and from what I gather, they can do a good job. I am wondering if
anyone has used "Slide Brite." What the sales rep tells me sounds
great, but can anyone speak from their own experience?
Thanks
Mike Todd
Skin Cancer Center of Northern Virginia
Sent by Medscape Mail: Free Portable E-mail for Professionals on the
Move
http://www.medscape.com
------------------------------
Message: 4
Date: Fri, 9 Jul 2004 12:21:01 -0700
From: "Shelkowsky, Ben" <Ben.Shelkowsky <@t> chomp.org>
Subject: [Histonet] DEZENKERIZE USING SAKURA DRS STAINER
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<384DA2BD670CE34DA8D3B60F4ED7157F4D612F <@t> exchsrvr.chomp.org>
Content-Type: text/plain; charset="utf-8"
I would like to dezenkerize my slides from mercury-fixed tissues on the
SAKURA automated stainer in a program I can internalize on the menu. Is
anyone out there already doing this and can you send me your procedure?
We would appreciate any help on this matter.
Ben Shelkowsky
CHOMP
Monterey, CA. 93950
ben.shelkowsky <@t> chomp.org
Confidentiality Notice:
This is a transmission from Community Hospital of the Monterey
Peninsula. This message and any attached documents may be confidential
and contain information protected by state and federal medical privacy
statutes. They are intended only for the use of the addressee. If you
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Thank you.
------------------------------
Message: 5
Date: Fri, 09 Jul 2004 15:36:53 -0400
From: "Fred Underwood" <funderwood <@t> mcohio.org>
Subject: Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s0eebb9c.061 <@t> mcohio.org>
Content-Type: text/plain; charset=US-ASCII
Set up a reagent station with idodine, 5 minutes shoud do. From there
just go through your rehydrating alcohols, tap water wash, and
continue
your staining procedure.
Fred
>>> "Shelkowsky, Ben" <Ben.Shelkowsky <@t> chomp.org> 07/09/04 03:21PM >>>
I would like to dezenkerize my slides from mercury-fixed tissues on
the
SAKURA automated stainer in a program I can internalize on the menu.
Is
anyone out there already doing this and can you send me your
procedure?
We would appreciate any help on this matter.
Ben Shelkowsky
CHOMP
Monterey, CA. 93950
ben.shelkowsky <@t> chomp.org
Confidentiality Notice:
This is a transmission from Community Hospital of the Monterey
Peninsula. This message and any attached documents may be
confidential
and contain information protected by state and federal medical privacy
statutes. They are intended only for the use of the addressee. If
you
are not the intended recipient, any disclosure, copying, or
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------------------------------
Message: 6
Date: Fri, 9 Jul 2004 17:06:19 -0400 (EDT)
From: "SMITH,REBEKAH FELICIA" <rockbeki <@t> ufl.edu>
Subject: Re: [Histonet] (no subject)
To: JAnorthernexp <@t> cs.com, Histonet <@t> lists.utsouthwestern.edu
Message-ID: <622625536.1089407179226.JavaMail.osg <@t> spnode33>
Content-Type: text/plain; format=flowed; charset=us-ascii
Which system?
On Wed Jul 07 04:35:02 EDT 2004, JAnorthernexp <@t> cs.com wrote:
> Hi there,
> Does anyone out there use this system and what is your procedure
> for cutting (micron thickness), drying time and temperature for
> slides or do you air dry using charge slides. And do you use a
> decloaker for deparaffinizing and antigen retrieval. thanks,
>
> Annette Hart, HT (ASCP)
> Wyoming Valley Health Care System
> Wilkes-Barre, PA _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the
stars
You have a right to be here and whether or not it is clear to you,
no doubt the universe is unfolding as it should. Therefore be at
peace with G-d, whatever you conceive Him to be. And whatever your
labors and aspirations,in the noisy confusion of life, keep peace
in your soul.-Max Ehrmann,"Desiderata"
------------------------------
Message: 7
Date: Fri, 9 Jul 2004 14:28:24 -0700
From: "Pereira, Laurie " <Laurie.Pereira <@t> sdcounty.ca.gov>
Subject: [Histonet] re: 266mp manual
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C075563B76F02A419AB6491FB86EE9AA06ABCC <@t> cosdi222.cosd.co.san-diego.ca.us>
Content-Type: text/plain; charset="iso-8859-1"
I have a manual for the fisher tissue processor 166/266mp. The manual
is approximately 59 pages long and I could make a copy. Just let me
know where to send it to.
Laurie Pereira
SDCADDL (Office of the County Veterinarian)
laurie.pereira <@t> sdcounty.ca.gov
------------------------------
Message: 8
Date: Fri, 9 Jul 2004 14:27:04 -0700
From: "Moklebust, Amanda C" <amoklebu <@t> seattlecca.org>
Subject: RE: [Histonet] B-Plus substitute for B5
To: "'Gladney, Diane C Ms MACH'" <Diane.Gladney <@t> se.amedd.army.mil>,
Joe Nocito <JNocito <@t> Pathreflab.com>, Histonet
<histonet <@t> pathology.swmed.edu>
Message-ID:
<5E6BFDF4F0AB2C4DA69CF4473FC7B948013CF306 <@t> wala01.seattlecca.org>
Content-Type: text/plain; charset="iso-8859-1"
It is good to hear that people are finding an adequate
replacement
for B-5. Before our lab can make the change the pathologists want to
see a
comparison study of at least two fixatives in addition to B-5. Can
anyone
pass on the names of other B-5 substitutes? Thanks for the help.
Amanda Moklebust, HTL
Pathology Laboratory
Seattle Cancer Care Alliance
Seattle, Washington
-----Original Message-----
From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney <@t> se.amedd.army.mil]
Sent: Thursday, July 08, 2004 1:12 PM
To: Joe Nocito; Histonet
Subject: RE: [Histonet] B-Plus substitute for B5
Joe,
We have been using B-Plus for about 2 years. Our pathologists love it
and
feel that it is equal to B-5. We have been required to eliminate the
use of
mercury products in our lab so this product has worked very exceptional
for
us. Trying to dispose of the mercury waste was getting to be a
nightmare.
Our pathologists only commented right after we started using this
product
that they noticed a very slight difference. They felt the difference
was
insignificant and adjusted quickly to the slight difference. Now they
say
that they can't tell the difference.....hmmm.
We had tried another product before discovering B-Plus that they just
hated.
I was thrilled that they accepted the B-Plus as I was out of options to
try
for a B-5 substitute.
Diane
Diane C. Gladney, HT (ASCP)
Histology /Cytology Supervisor
Moncrief Army Community Hospital
P.O. BOX 484
4500 Stuart Ave.
FT. Jackson, SC 29207
(803) 751-2530
DSN 734-2530
EMAIL: diane.gladney <@t> se.amedd.army.mil OR
dcgx1 <@t> aol.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]
Sent: Thursday, July 08, 2004 10:38 AM
To: Histonet
Subject: [Histonet] B-Plus substitute for B5
Good morning Histonetters,
My hempath is asking me to look into a substance called B-plus?
It's
supposed to replace B5. Any information will be greatly appreciated.
Thanks
Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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This electronic message transmission contains information which may
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or use of the contents of this information is prohibited. If you have
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delete this message. Opinions and ideas in this message that do not
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Notice of Privacy Practices, visit our web site at www.seattlecca.org.
------------------------------
Message: 9
Date: Fri, 09 Jul 2004 17:41:27 -0500
From: "ANN MARUSKA" <amarusk1 <@t> FAIRVIEW.ORG>
Subject: [Histonet] unsubscribe
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s0eed8cb.081 <@t> gw-northdom.fairview.org>
Content-Type: text/plain; charset=US-ASCII
please unsubscribe
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------------------------------
Message: 10
Date: Fri, 9 Jul 2004 15:59:14 -0700
From: "Wiese, Jason VHAROS" <Jason.Wiese <@t> med.va.gov>
Subject: RE: [Histonet] B-Plus substitute for B5
To: "'Moklebust, Amanda C'" <amoklebu <@t> seattlecca.org>,
"'Gladney,
Diane C Ms MACH'" <Diane.Gladney <@t> se.amedd.army.mil>, Joe
Nocito
<JNocito <@t> Pathreflab.com>,
Histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <A4C23F063576D311AEB90000F8312F2505264427 <@t> VHAROSEXC1>
Content-Type: text/plain; charset="iso-8859-1"
I use Presto-fixe II(B-5 replacement). I purchase it from Spectra
Tint
(Phone 800-888-8468... Fax 716-546-7979) Item #F112 It may not work
for
your needs, depending on what they are, but it is a good product to use
in a
comparison study.
Jason Wiese, HT(ASCP)
VHAROS Histology
913 Garden Valley Blvd
Roseburg, OR 97470
(541)-440-1000 x44751
-----Original Message-----
From: Moklebust, Amanda C [mailto:amoklebu <@t> seattlecca.org]
Sent: Friday, July 09, 2004 2:27 PM
To: 'Gladney, Diane C Ms MACH'; Joe Nocito; Histonet
Subject: RE: [Histonet] B-Plus substitute for B5
It is good to hear that people are finding an adequate
replacement
for B-5. Before our lab can make the change the pathologists want to
see a
comparison study of at least two fixatives in addition to B-5. Can
anyone
pass on the names of other B-5 substitutes? Thanks for the help.
Amanda Moklebust, HTL
Pathology Laboratory
Seattle Cancer Care Alliance
Seattle, Washington
-----Original Message-----
From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney <@t> se.amedd.army.mil]
Sent: Thursday, July 08, 2004 1:12 PM
To: Joe Nocito; Histonet
Subject: RE: [Histonet] B-Plus substitute for B5
Joe,
We have been using B-Plus for about 2 years. Our pathologists love it
and
feel that it is equal to B-5. We have been required to eliminate the
use of
mercury products in our lab so this product has worked very exceptional
for
us. Trying to dispose of the mercury waste was getting to be a
nightmare.
Our pathologists only commented right after we started using this
product
that they noticed a very slight difference. They felt the difference
was
insignificant and adjusted quickly to the slight difference. Now they
say
that they can't tell the difference.....hmmm.
We had tried another product before discovering B-Plus that they just
hated.
I was thrilled that they accepted the B-Plus as I was out of options to
try
for a B-5 substitute.
Diane
Diane C. Gladney, HT (ASCP)
Histology /Cytology Supervisor
Moncrief Army Community Hospital
P.O. BOX 484
4500 Stuart Ave.
FT. Jackson, SC 29207
(803) 751-2530
DSN 734-2530
EMAIL: diane.gladney <@t> se.amedd.army.mil OR
dcgx1 <@t> aol.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]
Sent: Thursday, July 08, 2004 10:38 AM
To: Histonet
Subject: [Histonet] B-Plus substitute for B5
Good morning Histonetters,
My hempath is asking me to look into a substance called B-plus?
It's
supposed to replace B5. Any information will be greatly appreciated.
Thanks
Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This electronic message transmission contains information which may
be
confidential or privileged. The information is intended to be for the
use of the individual or entity named above. If you are not the
intended recipient, be aware that any disclosure, copying,
distribution
or use of the contents of this information is prohibited. If you have
received this electronic transmission in error, please leave a message
via telephone at (206) 288-6266, notify me by electronic reply, and
delete this message. Opinions and ideas in this message that do not
relate to official business are understood as neither given nor
endorsed by the Seattle Cancer Care Alliance. To view our complete
Notice of Privacy Practices, visit our web site at www.seattlecca.org.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Fri, 9 Jul 2004 17:19:04 -0700
From: "Luck, Greg D." <LuckG <@t> empirehealth.org>
Subject: RE: [Histonet] B-Plus substitute for B5
To: "'Wiese, Jason VHAROS'" <Jason.Wiese <@t> med.va.gov>, "'Moklebust,
Amanda C'" <amoklebu <@t> seattlecca.org>, "'Gladney, Diane C Ms
MACH'"
<Diane.Gladney <@t> se.amedd.army.mil>, Joe Nocito
<JNocito <@t> Pathreflab.com>,
Histonet <histonet <@t> pathology.swmed.edu>
Message-ID:
<EDAC013F7055E1438FF70C30C43A4BE2C87E8C <@t> exchange05.inhs.org>
Content-Type: text/plain; charset="iso-8859-1"
We use "Prefer" and/or "Z-Fix" as our B-5 replacements. Both are
manufactured by Anatech.
Greg Luck, BS, HT(ASCP)
Anatomic Pathology Supervisor
Deaconess Medical Center
800 W. 5th Ave
Spokane, WA 99204
Phone 509.473.7077
Fax 509.473.7133
luckg <@t> empirehealth.org
-----Original Message-----
From: Wiese, Jason VHAROS [mailto:Jason.Wiese <@t> med.va.gov]
Sent: Friday, July 09, 2004 3:59 PM
To: 'Moklebust, Amanda C'; 'Gladney, Diane C Ms MACH'; Joe Nocito;
Histonet
Subject: RE: [Histonet] B-Plus substitute for B5
I use Presto-fixe II(B-5 replacement). I purchase it from Spectra
Tint
(Phone 800-888-8468... Fax 716-546-7979) Item #F112 It may not work
for
your needs, depending on what they are, but it is a good product to use
in a
comparison study.
Jason Wiese, HT(ASCP)
VHAROS Histology
913 Garden Valley Blvd
Roseburg, OR 97470
(541)-440-1000 x44751
-----Original Message-----
From: Moklebust, Amanda C [mailto:amoklebu <@t> seattlecca.org]
Sent: Friday, July 09, 2004 2:27 PM
To: 'Gladney, Diane C Ms MACH'; Joe Nocito; Histonet
Subject: RE: [Histonet] B-Plus substitute for B5
It is good to hear that people are finding an adequate
replacement
for B-5. Before our lab can make the change the pathologists want to
see a
comparison study of at least two fixatives in addition to B-5. Can
anyone
pass on the names of other B-5 substitutes? Thanks for the help.
Amanda Moklebust, HTL
Pathology Laboratory
Seattle Cancer Care Alliance
Seattle, Washington
-----Original Message-----
From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney <@t> se.amedd.army.mil]
Sent: Thursday, July 08, 2004 1:12 PM
To: Joe Nocito; Histonet
Subject: RE: [Histonet] B-Plus substitute for B5
Joe,
We have been using B-Plus for about 2 years. Our pathologists love it
and
feel that it is equal to B-5. We have been required to eliminate the
use of
mercury products in our lab so this product has worked very exceptional
for
us. Trying to dispose of the mercury waste was getting to be a
nightmare.
Our pathologists only commented right after we started using this
product
that they noticed a very slight difference. They felt the difference
was
insignificant and adjusted quickly to the slight difference. Now they
say
that they can't tell the difference.....hmmm.
We had tried another product before discovering B-Plus that they just
hated.
I was thrilled that they accepted the B-Plus as I was out of options to
try
for a B-5 substitute.
Diane
Diane C. Gladney, HT (ASCP)
Histology /Cytology Supervisor
Moncrief Army Community Hospital
P.O. BOX 484
4500 Stuart Ave.
FT. Jackson, SC 29207
(803) 751-2530
DSN 734-2530
EMAIL: diane.gladney <@t> se.amedd.army.mil OR
dcgx1 <@t> aol.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]
Sent: Thursday, July 08, 2004 10:38 AM
To: Histonet
Subject: [Histonet] B-Plus substitute for B5
Good morning Histonetters,
My hempath is asking me to look into a substance called B-plus?
It's
supposed to replace B5. Any information will be greatly appreciated.
Thanks
Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This electronic message transmission contains information which may
be
confidential or privileged. The information is intended to be for the
use of the individual or entity named above. If you are not the
intended recipient, be aware that any disclosure, copying,
distribution
or use of the contents of this information is prohibited. If you have
received this electronic transmission in error, please leave a message
via telephone at (206) 288-6266, notify me by electronic reply, and
delete this message. Opinions and ideas in this message that do not
relate to official business are understood as neither given nor
endorsed by the Seattle Cancer Care Alliance. To view our complete
Notice of Privacy Practices, visit our web site at www.seattlecca.org.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Sat, 10 Jul 2004 06:14:54 +0500
From: "Muhammad Tahseen" <tahseen <@t> brain.net.pk>
Subject: Re: [Histonet] Room Temperature
To: "Lab Histologia" <histolog <@t> fcv.unl.edu.ar>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <008001c4661b$50e8ca00$972bfea9 <@t> User>
Content-Type: text/plain; charset="iso-8859-1"
25c
----- Original Message -----
From: Lab Histologia <histolog <@t> fcv.unl.edu.ar>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, July 08, 2004 7:01 PM
Subject: [Histonet] Room Temperature
> Hello, everyone,
>
> I need know that range of temperature defines you as room
temperature.
>
> Thanks
>
>
> Hugo
>
> -------------------------------------------------------------------
>
> Dr. Hugo H. Ortega (DMV, PhD)
>
> Departament of Cellular Biology
>
> Faculty of Veterinary Sciences
>
> Universidad Nacional del Litoral
>
> R.P. Kreder 2805 - Esperanza (3080)
>
> Santa Fe - ARGENTINA
>
> Tel. (54)3496-420639
>
> Fax. (54)3496-426304
>
> http://fcv.unl.edu.ar/histolog/
>
> http://fcv.unl.edu.ar/bioterio/
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 13
Date: Sat, 10 Jul 2004 05:57:51 -0400
From: <lpwenk <@t> sbcglobal.net>
Subject: Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER
To: "Fred Underwood" <funderwood <@t> mcohio.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003c01c46664$5d32f580$9f27d445 <@t> domainnotset.invalid>
Content-Type: text/plain; charset="iso-8859-1"
Reminder to everyone who is dezenkerizing (lugolizing) on the stainers
-
when you get ready to dump this iodine station, it cannot go down the
sink.
It now contains mercuric salts, so must be collected as mercury waste.
(As
should the next couple of containers of reagents, carry-over on the
slides.)
Ways to get around this:
1. Dezenkerize (lugolize) by hand, then add the slide to the stainer.
2. Switch over to a zinc fixative.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
----- Original Message -----
From: "Fred Underwood" <funderwood <@t> mcohio.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, July 09, 2004 3:36 PM
Subject: Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER
> Set up a reagent station with idodine, 5 minutes shoud do. From
there
> just go through your rehydrating alcohols, tap water wash, and
continue
> your staining procedure.
>
> Fred
>
> >>> "Shelkowsky, Ben" <Ben.Shelkowsky <@t> chomp.org> 07/09/04 03:21PM
>>>
> I would like to dezenkerize my slides from mercury-fixed tissues on
the
> SAKURA automated stainer in a program I can internalize on the menu.
Is
> anyone out there already doing this and can you send me your
procedure?
>
> We would appreciate any help on this matter.
>
> Ben Shelkowsky
> CHOMP
> Monterey, CA. 93950
> ben.shelkowsky <@t> chomp.org
> Confidentiality Notice:
> This is a transmission from Community Hospital of the Monterey
> Peninsula. This message and any attached documents may be
confidential
> and contain information protected by state and federal medical
privacy
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> Thank you.
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>
>
>
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------------------------------
Message: 14
Date: Sat, 10 Jul 2004 06:08:34 -0400
From: <lpwenk <@t> sbcglobal.net>
Subject: Re: [Histonet] Room Temperature
To: "Muhammad Tahseen" <tahseen <@t> brain.net.pk>, "Lab Histologia"
<histolog <@t> fcv.unl.edu.ar>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <004501c46665$dcc2cae0$9f27d445 <@t> domainnotset.invalid>
Content-Type: text/plain; charset="iso-8859-1"
Take the temperature of YOUR room, and use that.
Muhammad said 25 degrees C.
25 degrees C = 77 degrees F
That would be too hot in our lab in Michigan, which is air conditioned
in
the spring/summer/fall, and never too hot when the furnace is on in
the
winter. Plus, with the increased air ventilation/flow, it always seems
chillier.
22 degrees C = 71.6 degrees F.
20 degrees C = 68 degrees F.
That's more the range in our anatomic pathology labs.
Of course, one year they move the student lab into a room that the
heat
didn't work very well. We did have a large picture window, but there
was a
sheet of ice on it all winter long. Our average that winter was:
15 degrees C = 59 degrees F
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
----- Original Message -----
From: "Muhammad Tahseen" <tahseen <@t> brain.net.pk>
To: "Lab Histologia" <histolog <@t> fcv.unl.edu.ar>;
<histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, July 09, 2004 9:14 PM
Subject: Re: [Histonet] Room Temperature
> 25c
> ----- Original Message -----
> From: Lab Histologia <histolog <@t> fcv.unl.edu.ar>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Thursday, July 08, 2004 7:01 PM
> Subject: [Histonet] Room Temperature
>
>
> > Hello, everyone,
> >
> > I need know that range of temperature defines you as room
temperature.
> >
> > Thanks
> >
> >
> > Hugo
> >
> >
-------------------------------------------------------------------
> >
> > Dr. Hugo H. Ortega (DMV, PhD)
> >
> > Departament of Cellular Biology
> >
> > Faculty of Veterinary Sciences
> >
> > Universidad Nacional del Litoral
> >
> > R.P. Kreder 2805 - Esperanza (3080)
> >
> > Santa Fe - ARGENTINA
> >
> > Tel. (54)3496-420639
> >
> > Fax. (54)3496-426304
> >
> > http://fcv.unl.edu.ar/histolog/
> >
> > http://fcv.unl.edu.ar/bioterio/
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Sat, 10 Jul 2004 07:37:57 -0500
From: "Winters, Bert" <BWinters <@t> NCH.ORG>
Subject: [Histonet] PROSTATE BIOSIES
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <270614B321ACB44D8C1D91F4F921FDC362776D <@t> NCH01EX02.nch.org>
Content-Type: text/plain; charset="iso-8859-1"
I am looking for any suggestions when it comes to processing prostate
biopsies. The pathologist have going back and forth with processing
these biopsies using a typical biopsy program on the tissue processors
and using a routine process which is used for processing most tissue
specimens. Everyone I have talked with about this processes all prostate
biopsies using a biopsy program, about 5-6 hours in the tissue
processor. Any processing or staining tips anyone could give me about
prostate biopsies would be greatly appreciated.
Bert
------------------------------
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End of Histonet Digest, Vol 8, Issue 16
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