[Histonet] Diastase digestion

[Angela Bitting]

I've got a head-scratcher here.
We stained three human liver cases with  PAS w/ diastase. We mount our patient tissue on the same slide below the control tissue. We do the diastase digestion in a Coplin jar and all the slides were in the same jar.
Our Pathologist showed me the slides. The glycogen was removed from the control tissue, but the test tissue was still positive.
Does anyone have an idea what happened?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Laboratories
(570)214-9634


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