[Histonet] fixative for giemsa stain?

[Marshall Terry Dr, Consultant Histopathologist]

Although the cells will fix as they stain, with the alcohol in the stain, the right thing to do is to fix in alcohol beforehand. 20 minutes in methanol is the usually quoted time for best results. The purists insist on methanol, though I have never been able to see the difference between that an ethanol. My and most others experience is with blood and marrow, but I guess a Giemsa is a Giemsa whatsoever it is staining.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall <@t> rothgen.nhs.uk

-----Original Message-----
From: Julia Edgar [mailto:je22r <@t> udcf.gla.ac.uk]
Sent: 08 July 2004 15:55
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] fixative for giemsa stain?


Dear All
I need to do a giemsa stain on some primary muscle cells. Can you tell me if I should fix the cells first, and if so, with which fixative. Thank you.
Julia
Julia Edgar (BSc, PhD)
University of Glasgow Veterinary School
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