[Histonet] Ideal endothelial cell-isolatin' antibody

[Patsy Ruegg]

Jim,
Your best bet would be CD31, other alternatives would include cd34, factor
VIII, and vegf, but cd31 is probably the most specific for just endothelial
cells, the others stain a lot of other things as well.  We didn't get
endoglin to work very well in ffpe tissue, it was inconsistant.  You should
be able to biotinylate or buy a bt cd31 and do a quick sensitive detection
for it.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Gagermeier, James
Sent: Tuesday, January 27, 2004 9:41 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Ideal endothelial cell-isolatin' antibody




I am attempting to find an antibody for immunofluorescence that is the best
(from experience) in isolating endothelial cells.  This is significant for
me as I am attempting to isolate endothelail cells in order to perform laser
microdissection on these cells for further studies.  The problem I am faced
with is that an approach utilizing double staining will take too long,
allowing RNA degradation.  It is reccomended that a specific primary
biotinylated antibody be used - and then a rapid IF staining kit by Arcturus
is used.  The IF protocol appears to take 12-15 minutes.

I am trying to determine the best antibody so that I can use a single
biotinylated primary antibody with the rapid Arcturus IF protocol.  Any
reccs are appreciated.

Thanks

Jim Gagermeier

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