[Histonet] Articular cartilage wrinkles

[Gehan, Loralee]

We cut our samples at 3microns.  This seems to help.  And we have also
started using a slide warmer.  Set at about 37C.  Lay the slides on that
after sectioning and let sit for a few hours or all day.  Depending on your
preference.  This slide warmer has improved the way the sections stick to
the slides and also eliminated the wrinkles.  
Our slide warmer came from Fisher Scientific.  Around $500, but well worth
it.  We bought two.  
Hope that helps you. 

Loralee Gehan
Orthopaedics Research lab

> ----------
> From: 	Wadsworth, Marilyn P
> Sent: 	Wednesday, January 28, 2004 9:51 AM
> To: 	histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] Articular cartilage wrinkles
> 
> 
> Dear Histonet,
> 
> > I am processing specimens of articular cartilage/bone from rabbit
> > specimens.  The tissue has been fixed and decaled using EDTA in
> > formalin using the method of Kirviranta, 1985.  
> > 
> > Kiviranta, I., et al., Microspectrophotometric quantitation of
> > glycosaminoglycans in articular cartilage sections stained with
> > Safranin O. Histochemistry., 1985. 82(3): p. 249-55.
> > 
> > The sections are paraffin embedded and sectioned at 5 or 6 microns.
> > Upon mounting on slides, the articular cartilage appears puckered or
> > wrinkled (result of swelling of cartilage when exposed to water).   Is
> > there anything that may be done to eliminate this artifact?  Is it
> > possible to float the microtomed sections on a non-aqueous solution?
> > I'd appreciate any tips you may offer.  Thanks
> > 
> Maria Roemhildt, PhD
> University of Vermont
> Department of Orthopaedics & Rehabilitation
> 95 Carrigan Drive
> Burlington, VT  05405
> 
> Ph. 802-656-3823
> fax:  802-656-4247
> 
> maria.roemhildt <@t> med.uvm.edu
> 
> 
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