[Histonet] combined ISH/IHC

[Tora Bardal]

Hello
Does anyone have a working protocol they can share?

I'm trying to combine in situ hybridization (RNAprobe) and 
immunohistochemistry (PCNA) on fish paraffin sections.
So far: ISH ok, IHC doesn't work, or ISH no signal, IHC ok. PCNA(DAKO) 
needs AR.

Before spending more time an money I would like to have some good advices 
on what to do when, ab simultaneous incubation? stepwise? crossreactions? 
blocking ?
I'm using sheep anti-DIG-AP/BCIP/NBT (ISH) and DAKO Envision HRP (mouse)/DAB.

Tora


Tora Bardal
Department of Biology,
Norwegian University of Science and Technology (NTNU)
Brattøra Research Center
N-7491 Trondheim
Norway                                   + (47)73 59 09 38 / 970 25256
E-mail: Tora.Bardal <@t> bio.ntnu.no         fax:+ (47)73 59 63 11