[Histonet] Articular cartilage wrinkles

[Wadsworth, Marilyn P]

Dear Histonet,

> I am processing specimens of articular cartilage/bone from rabbit
> specimens.  The tissue has been fixed and decaled using EDTA in
> formalin using the method of Kirviranta, 1985.  
> 
> Kiviranta, I., et al., Microspectrophotometric quantitation of
> glycosaminoglycans in articular cartilage sections stained with
> Safranin O. Histochemistry., 1985. 82(3): p. 249-55.
> 
> The sections are paraffin embedded and sectioned at 5 or 6 microns.
> Upon mounting on slides, the articular cartilage appears puckered or
> wrinkled (result of swelling of cartilage when exposed to water).   Is
> there anything that may be done to eliminate this artifact?  Is it
> possible to float the microtomed sections on a non-aqueous solution?
> I'd appreciate any tips you may offer.  Thanks
> 
Maria Roemhildt, PhD
University of Vermont
Department of Orthopaedics & Rehabilitation
95 Carrigan Drive
Burlington, VT  05405

Ph. 802-656-3823
fax:  802-656-4247

maria.roemhildt <@t> med.uvm.edu