[Histonet] lifting tissue!

[BRobert <@t> ameripath.com]

I had similar problems with thicker stented artery sections and we solved it
by leaving slides overnight in xylene at ~60 degrees. You might want to give
it a try.
Good luck!
Brigitte 

-----Original Message-----
From: Donna Teleis [mailto:dteleis <@t> vet.upenn.edu]
Sent: Friday, January 23, 2004 2:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] lifting tissue!


Okay, so here is my problem...

I am cutting Equine Intestine, in MMA, at 3.5 microns, using a Tungsten
blade. 
No problems there. I float them on a drop of 30% ETOH on a slide warmer set
at 
50 degrees C, and they flatten out nicely. They sit on the warmer overnight.

To remove the plastic, I put the slides in warm (40 deg) xylene for 60 
minutes, then rinse with 70% ETOH, 50% ETOH, to dist. water. All of this
seems 
to work well, and the tissue sticks on the slide like glue, except for the 
mucosa. For some reason, when the stained slide dries (H&E stain)the mucosal

layer lifts. The rest of the tissue is fine, but that little rim of mucosa 
just folds right up from the slide.

Any thoughts? Ideas?

These sections are heading for IHC, and while I know they will survive all
the 
steps while wet, I dont want to loose them when they dry at the end!!

Thanks
Donna

Donna Teleis
Research Specialist
Department of Clinical Studies
Equine Sports Medicine Building
University of Pa., New Bolton Center
phone: 610-925-6429 
fax: 610-925-8131


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