[Histonet] lifting tissue!

[Donna Teleis]

Okay, so here is my problem...

I am cutting Equine Intestine, in MMA, at 3.5 microns, using a Tungsten blade. 
No problems there. I float them on a drop of 30% ETOH on a slide warmer set at 
50 degrees C, and they flatten out nicely. They sit on the warmer overnight. 
To remove the plastic, I put the slides in warm (40 deg) xylene for 60 
minutes, then rinse with 70% ETOH, 50% ETOH, to dist. water. All of this seems 
to work well, and the tissue sticks on the slide like glue, except for the 
mucosa. For some reason, when the stained slide dries (H&E stain)the mucosal 
layer lifts. The rest of the tissue is fine, but that little rim of mucosa 
just folds right up from the slide.

Any thoughts? Ideas?

These sections are heading for IHC, and while I know they will survive all the 
steps while wet, I dont want to loose them when they dry at the end!!

Thanks
Donna

Donna Teleis
Research Specialist
Department of Clinical Studies
Equine Sports Medicine Building
University of Pa., New Bolton Center
phone: 610-925-6429 
fax: 610-925-8131