[Histonet] (no subject)

[Gagermeier, James]

Basics of LCM 

A few questions in regards to laser capture microdissection utilizing a
Leica AS LMD System:

In regards to buffers to place in the caps - I have routinely used RNA
later. This, however, readily crystalizes setting up issues with debris on
the microscope. First, is this an acceptable buffer, and second, what are
some other buffer alternatives.

Furthermore, when preparing slides, I generally store them in a -80 C unit.
I generally place them in a zip lock bag - not a heat sealed bag. Also, I do
not generally add a dessicant. Should:

a) I use heat-sealed bags. If not, do I need to place them in a zip lock at
all ?(i.e. can I simply put them in a slide box in the -80C unit)

b) Do I need to start using a dessicant?

I appreciate any response on these issues.  


Jim Gagermeier