[Histonet] (no subject)

[Kathleen Spencer]

I put slides in a slide box with dessicant, wrap the box in foil, and 
place this in a ziploc bag.
Then into the  -80. I am sure you know never to let them air dry. I have 
an Arcturus LCM and we mostly use their kits, thus their buffers.

Kathleen Spencer
LCM Supervisor
UTHSC


On Thursday, January 22, 2004, at 12:56  AM, Gagermeier, James wrote:

> Basics of LCM
>
> A few questions in regards to laser capture microdissection utilizing a
> Leica AS LMD System:
>
> In regards to buffers to place in the caps - I have routinely used RNA
> later. This, however, readily crystalizes setting up issues with debris 
> on
> the microscope. First, is this an acceptable buffer, and second, what 
> are
> some other buffer alternatives.
>
> Furthermore, when preparing slides, I generally store them in a -80 C 
> unit.
> I generally place them in a zip lock bag - not a heat sealed bag. Also, 
> I do
> not generally add a dessicant. Should:
>
> a) I use heat-sealed bags. If not, do I need to place them in a zip 
> lock at
> all ?(i.e. can I simply put them in a slide box in the -80C unit)
>
> b) Do I need to start using a dessicant?
>
> I appreciate any response on these issues.
>
>
> Jim Gagermeier
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