[Histonet] Pecam-1 (CD31)

[Jackie.O'Connor <@t> abbott.com]

Novocastra  NCL-CD31-1A10.  Citrate 6.0pH HIER - works well on formalin 
fixed murine tissue.

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics





Angela McNabola <angela.mcnabola.b <@t> bayer.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
01/20/2004 11:07 AM

 
        To:     Loralee_Gehan <@t> URMC.Rochester.edu, histonet <@t> pathology.swmed.edu
        cc: 
        Subject:        Re: [Histonet] Pecam-1 (CD31)






Hi,

We use Santa Cruz PECAM-1 M-20 (SC-15060) goat polyclonal.

We steam for 30 minutes in Dako TRS (S1699) for 30 minutes (cool to room
temperature).

We also avidin/biotin block (Vector), and use ABC (Vector elite kit).

Works great, especially on mouse xenografts.

Let me know if you need more information.

Angela McNabola, MS, HT(ASCP)SLS
Bayer Corp.
West Haven, CT


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