[Histonet] C4d antibody

[Stapf, Ross]

We also use the Biogenisis C4d for Frozen.

For Paraffin sections we use Alpco Diagnostics CAT #004-BI-RC4D
(www.alpco.com) at 1:40.  Pretreatment is citrate buffer (pH 6.0) in the
pressure cooker for 10 minutes.  We use the Envision + detection system
from Dako.

Ross M Stapf
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465 
214-820-4110 fax
RossS <@t> baylorhealth.edu



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree
Linda A.
Sent: Tuesday, January 20, 2004 1:17 PM
To: Featherstone, Annette; Instrumedics; HistoNet Server
Subject: RE: [Histonet] C4d antibody


Annette,

We use C4d on frozen renal biopsies at 1:600 for 32" on Ventana
automated staining instruments after 2" in Ventana's Morphosave.  This
is a DAB protocol.  Our C4d is from Biogenesis, cat. # 2222-8004.  

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: Featherstone, Annette [mailto:AFeatherstone <@t> KaleidaHealth.Org]
Sent: Tuesday, January 20, 2004 12:41 PM
To: 'Instrumedics'; HistoNet Server
Subject: [Histonet] C4d antibody


Hi

Anyone out there using C4d antibody? Have to work it up and want to know
if there are any tips to make this work as they haven't been able to get
it to work right. Manufacturer recommends Alk Phos, but I was hoping to
get it to work with DAB. Any suggestions? Thanks Annette Featherstone
HT/MLT




-----Original Message-----
From: Instrumedics [mailto:info <@t> instrumedics.com]
Sent: Tuesday, January 20, 2004 12:44
To: HistoNet Server
Subject: [Histonet] tissue carryover


One of the advantages of the Cryo-Vac-Away vacuum system for the
cryostat is that the debris generated during the trimming step is
suctioned away as  it is generated at the blockface. This substantially
reduces the chances that any sections will cling to the underside of the
knife which can then can carryover to the following case. 

Bernice
schiller <@t> instrumedics.com



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