[Histonet] Proteolytic enzyme....Dako replacement

Carl carl.hobbs <@t> kcl.ac.uk
Tue Jan 20 13:05:14 CST 2004

Be very grateful for any input.....Dako used to do a trypsin soln that was great for my BrdU disclosing immunostaining. They no longer provide this but now market a replacement, S 3007. This gives relatively poor disclosure over a range of incubation times eg 5-30mins. Additionally, the tissue, even at 10mins incubation( at room temp!), appears rather digested. ( did H&E on sequential section and that showed no evidence of proteolytic digestion)Thanks

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