[Histonet] Pecam-1 (CD31)

[Gayle Callis]

The antibody is available from BD Pharmingen and probably SEROTEC, but we
only work with this marker on frozen sections. It is a monoclonal, rat
antimouse.  

Check out the spec sheets from BD Pharmingen, they should tell you whether
or not the antibody works on FFPE tissues. There is probably info already
in Histonet archives, so do a search for whether the antibody works on
FFPE, and retrieval method. 

At 11:45 AM 1/20/2004 -0500, you wrote:
>Has anyone out there come across a CD31 that works in formalin fixed
>paraffin embedded mouse tissue?  Any retrieval methods used would help also.
>
>There are about 200 CD31's for human tissue.  Can't seem to find one that
>works in mouse. 
>
>Thanks a lot.
>
>
>Loralee Gehan
>Orthopaedics Research Lab
>
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>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)