[Histonet] C4d antibody

Tue Jan 20 13:17:04 CST 2004

Annette,

We use C4d on frozen renal biopsies at 1:600 for 32" on Ventana automated staining instruments after 2" in Ventana's Morphosave.  This is a DAB protocol.  Our C4d is from Biogenesis, cat. # 2222-8004.  

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174

-----Original Message-----
From: Featherstone, Annette [mailto:AFeatherstone <@t> KaleidaHealth.Org]
Sent: Tuesday, January 20, 2004 12:41 PM
To: 'Instrumedics'; HistoNet Server
Subject: [Histonet] C4d antibody

Hi

Anyone out there using C4d antibody? Have to work it up and want to know if
there are any tips to make this work as they haven't been able to get it to
work right. Manufacturer recommends Alk Phos, but I was hoping to get it to
work with DAB. Any suggestions? Thanks
Annette Featherstone HT/MLT

-----Original Message-----
From: Instrumedics [mailto:info <@t> instrumedics.com]
Sent: Tuesday, January 20, 2004 12:44
To: HistoNet Server
Subject: [Histonet] tissue carryover

One of the advantages of the Cryo-Vac-Away vacuum system for the cryostat is
that the debris generated during the trimming step is suctioned away as  it
is generated at the blockface. This substantially reduces the chances that
any sections will cling to the underside of the knife which can then can
carryover to the following case. 

Bernice
schiller <@t> instrumedics.com

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