[Histonet] C4d antibody

[Andi Kappeler]

Hi Annette

rb-a-C4d, Biomedica (http://www.biomedica.co.at/; in the US try
http://www.bmgrp.com/) Cat.No. BI-RC4D. Working dilution around 1:25-1:50.
Requires HIER, pressure cooker with citrate buffer, pH 6.0, may be okay,
better results with microwave and 100 mM Tris - 5% urea, pH 9.5 (strange
stuff, but it works). Make sure to use a biotin-independent visualization
system or use an avidin-biotin block. Good luck!
Regards,

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

----- Original Message ----- 
From: "Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org>
To: "'Instrumedics'" <info <@t> instrumedics.com>; "HistoNet Server"
<HistoNet <@t> Pathology.swmed.edu>
Sent: Tuesday, January 20, 2004 7:41 PM
Subject: [Histonet] C4d antibody


> Hi
>
> Anyone out there using C4d antibody? Have to work it up and want to know
if
> there are any tips to make this work as they haven't been able to get it
to
> work right. Manufacturer recommends Alk Phos, but I was hoping to get it
to
> work with DAB. Any suggestions? Thanks
> Annette Featherstone HT/MLT