[Histonet] Frozen section ice artifact

[Patsy Ruegg]

Jackie,
I am having the same kind of trouble with some mishandled frozen heart
samples.  I was told that Vinnie and Rena Fall wrote an article for
Histologic in ?May 03 on how to repair poorly frozen samples but I spent a
lot of time trying to see the article on the Sakura web site this weekend
without success.  Can someone tell me how to fix this heart sample or what
was in their article please.  I think it has something to do with melting
the block and refreezing it properly.  Jackie I would like to see your
sample to see if it resembles mine.  I cannot recognize my tissue as muscle
it is all shredded with spaces etc.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Jackie.O'Connor <@t> abbott.com
Sent: Monday, January 12, 2004 11:08 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Frozen section ice artifact


Yo Histonetters, and Happy New Year.

I just cut some frozen sections of solid tumors in OCT which were supposed
to be snap frozen in liquid nitrogen after being put in plastic cryomolds.
I have never seen such horrible morphology in my career.  I think this is
an example of the absolute worst ice crystal artifact I have ever seen,
but since I have never seen anything this bad before, I don't know what
I'm seeing, you see?    I hope I've intrigued someone enough to email me
directly, so I can send you a gif file of this atrocity.  I think the
tissues were mishandled prior to freezing (someone else froze them) and
I'm trying to figure out what happened so I can correct the problem.  I'd
appreciate anyone who would like to take a look at this artifact - it's
awful, honest!

Jackie O'Connor
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