[Histonet] Frozen section ice artifact

[LuAnn Anderson]

Hi Patsy and Jackie,
I have the article and would be happy to fax a copy to anyone who needs it. 
If you'd like me to fax a copy to you, please email me with your fax number 
and I will get it right off to you.
LuAnn Anderson HT(ASCP)
Neuropathology Lab
University of Minnesota


At 12:43 PM 1/12/04 -0700, Patsy Ruegg wrote:
>Jackie,
>I am having the same kind of trouble with some mishandled frozen heart
>samples.  I was told that Vinnie and Rena Fall wrote an article for
>Histologic in ?May 03 on how to repair poorly frozen samples but I spent a
>lot of time trying to see the article on the Sakura web site this weekend
>without success.  Can someone tell me how to fix this heart sample or what
>was in their article please.  I think it has something to do with melting
>the block and refreezing it properly.  Jackie I would like to see your
>sample to see if it resembles mine.  I cannot recognize my tissue as muscle
>it is all shredded with spaces etc.
>Patsy
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
>Jackie.O'Connor <@t> abbott.com
>Sent: Monday, January 12, 2004 11:08 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Frozen section ice artifact
>
>
>Yo Histonetters, and Happy New Year.
>
>I just cut some frozen sections of solid tumors in OCT which were supposed
>to be snap frozen in liquid nitrogen after being put in plastic cryomolds.
>I have never seen such horrible morphology in my career.  I think this is
>an example of the absolute worst ice crystal artifact I have ever seen,
>but since I have never seen anything this bad before, I don't know what
>I'm seeing, you see?    I hope I've intrigued someone enough to email me
>directly, so I can send you a gif file of this atrocity.  I think the
>tissues were mishandled prior to freezing (someone else froze them) and
>I'm trying to figure out what happened so I can correct the problem.  I'd
>appreciate anyone who would like to take a look at this artifact - it's
>awful, honest!
>
>Jackie O'Connor
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