Fwd: [Histonet] Mast Cells Stain

[Atoska S. Gentry]

Hello, we too use Toluidine Blue Stain (TBO) for mast cells. After 
deparaffinization & hydrating, stain in 0.1% Toluidine Blue Stain for 10 
seconds - 1 minute depending on stain (until tissue is medium blue in 
color). 10 seconds works best for us. After staining rinse in distilled 
water, dehydrate, clear, & mount. Best wishes. Atoska

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>Subject: [Histonet] Does anyone know of a quick methylene blue stain for mast
>         cells?  It rings a bell with me, but can't
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>Does anyone know of a quick methylene blue stain for mast cells?  It rings 
>a bell with me, but can't put a finger on it or find it in any of our 
>reference literature. hay-ulp!!!
>
>thanks in advance
>
>
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Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850