[Histonet] Need good S-100 MAb for paraffin sections

d.gregg <@t> juno.com d.gregg <@t> juno.com
Fri Feb 6 13:23:05 CST 2004


Can anyone suggest a strong and robust MAb for S-100 that will work in
formalin fixed or PLP fixed tissue embedded in paraffin. I have used a
polyclonal rabbit antibody that works OK but I am using pig tissues and
get too much background with a rabbit antibody despite various blocking
regimes and adsorption of the secondary with swine serum. Thanks for any
suggestions.

Douglas Gregg
Plum Island Animal Disease Center
Greenport, NY 




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