[Histonet] gliut/isopropyl alcohol[Scanned]

[Molinari, Betsy]

Hi Kemlo,
Thanks for your suggestion, I did not think of rinsing! The EM tech here
had never heard of a glut/isopropyl mix, we use a 2%glut solution in
buffer for the EM done here. I will be cutting and staining the blocks
today, it will be interesting.
Thanks,
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
Houston, TX 77373
832-355-6524

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kemlo
Rogerson
Sent: Wednesday, December 01, 2004 2:25 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] gliut/isopropyl alcohol[Scanned]

Expect the tissue is rather brittle, used to be used for
electromicroscopy I
think. Wonder if you could secondary fix? Can't aldehyde/ alcohol
fixation
be 'washed out' then the tissue refixed in something more conventional? 

-----Original Message-----
From: Molinari, Betsy [mailto:BMolinari <@t> heart.thi.tmc.edu] 
Sent: 30 November 2004 19:08
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] gliut/isopropyl alcohol[Scanned]

Hi histonetters,
 I received samples that have been fixed   in a   1%glut / 20% isopropyl
alcohol solution for paraffin processing. I have never heard of this
fixative, I know alcoholic formalin but never alcoholic glutaraldehyde.
Are any of you familiar with this fixative?
Thank you.
Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
Houston,TX 77030
832-355-6524
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