[Histonet] gliut/isopropyl alcohol

[Molinari, Betsy]

Hi Bob,
Thanks for your input. I may ask a couple of the surgery staff here if
they are familiar with that technique. A point of interest, these are
valves. I loaded them last night starting in 70% ETOH, I am curious to
see how they turned out and how much effect it will have on the B&B and
PTAH I will be running on them
Thanks again,
Betsy Molinari  HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
Houston, TX 77030
832-355-6524
 
-----Original Message-----
From: RCHIOVETTI <@t> aol.com [mailto:RCHIOVETTI <@t> aol.com] 
Sent: Tuesday, November 30, 2004 6:26 PM
To: Molinari, Betsy; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] gliut/isopropyl alcohol
 
In a message dated 11/30/2004 12:12:08 PM US Mountain Standard Time,
BMolinari <@t> heart.thi.tmc.edu writes:
	I received samples that have been fixed   in a   1%glut / 20%
isopropyl
	alcohol solution for paraffin processing. I have never heard of
this
	fixative, I know alcoholic formalin but never alcoholic
glutaraldehyde.
	Are any of you familiar with this fixative?
	Thank you.
	Betsy Molinari HT (ASCP)
Hi Betsy,
 
Is the specimen perhaps a heart valve made from pig tissue??
 
The only time I've heard of alcoholic glutaraldehyde is for pretreating
pig valves before they're implanted in humans.  There's some kind of
relationship between alcohol and glut, and preventing calcification of
the valve.  Can't remember the details right now, but you have to treat
the valve with one of those reagents before using the other.
 
Just a thought...
 
Cheers,
 
Bob Chiovetti