[Histonet] Successful frozen sections on bone
gcallis <@t> montana.edu
Fri Aug 27 11:32:49 CDT 2004
The ONLY way we have successful cryosections of any kind of bone is using
the Instrumedics CryoJane tape transfer system. Check out their website for
information, how it works.
Bone frozen sections are destroyed by disposable or c profile steel blade,
tungsten carbide blades are ideal as you are finding out. However, I have
never had a bone frozen section cut with a TC knife which remains intact
unless the tape transfer method is used to maintains bone
integrity. Cutting temperature must be -28C up to -32C or so.
Your tungsten carbide knife edge must be in perfectly, sharp condition even
with tape transfer method.
For us, cryosectioning bone without the Cryojane has been a waste of
time. Although a pricey bit of equipment, it has paid for itself many
times over with good bone frozen sections.
I am going to attach a publication to you privately so you can see advantages.
At 10:02 AM 8/27/2004, you wrote:
>I am doing frozen sections on fresh, undecalcified mouse femurs. My
>sections seem to be losing all of the marrow and many are torn . I have
>tried disposable cryostat knives as well as the tungsten carbide, but the
>sections are still not good. If anyone else is doing this procedure, could
>you share any tips or suggestions on your technique, cutting temperatures,
>or overall procedure. I have tried different temperatures, but none of them
>seem to give me the sections I want. Any help would
>be greatly appreciated.
>Nancy.Lowen <@t> med.va.gov
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>Histonet <@t> lists.utsouthwestern.edu
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