[Histonet] Successful frozen sections on bone

Gayle Callis gcallis <@t> montana.edu
Fri Aug 27 11:32:49 CDT 2004

The ONLY way we have successful cryosections of any kind of bone is using 
the Instrumedics CryoJane tape transfer system. Check out their website for 
information, how it works.
Bone frozen sections are destroyed by disposable or c profile steel blade, 
tungsten carbide blades are ideal as you are finding out. However, I have 
never had a bone frozen section cut with a TC knife which remains intact 
unless the tape transfer method is used to maintains bone 
integrity.  Cutting temperature must be -28C up to -32C or so.

Your tungsten carbide knife edge must be in perfectly, sharp condition even 
with tape transfer method.

For us, cryosectioning bone without the Cryojane has been a waste of 
time.  Although a pricey bit of equipment,  it has paid for itself many 
times over with good bone frozen sections.

I am going to attach a publication to you privately so you can see advantages.

At 10:02 AM 8/27/2004, you wrote:
>I am doing frozen sections on fresh, undecalcified mouse femurs.  My
>sections seem to be losing all of the marrow and many are torn .  I have
>tried disposable cryostat knives as well as the tungsten carbide, but the
>sections are still not good.  If anyone else is doing this procedure, could
>you share any tips or suggestions on your technique, cutting temperatures,
>or overall procedure. I have tried different temperatures, but none of them
>seem  to give me the sections I want.  Any help would
>be greatly appreciated.
>Nancy.Lowen <@t> med.va.gov
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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