[Histonet] frozen sections on bone

[Lowen, Nancy]

I am doing frozen sections on fresh, undecalcified mouse femurs.  My
sections seem to be losing all of the marrow and many are torn .  I have
tried disposable cryostat knives as well as the tungsten carbide, but the
sections are still not good.  If anyone else is doing this procedure, could
you share any tips or suggestions on your technique, cutting temperatures,
or overall procedure. I have tried different temperatures, but none of them
seem  to give me the sections I want.  Any help would  

be greatly appreciated.

Nancy.Lowen <@t> med.va.gov