[Histonet] CD3 on BM

[Sebree Linda A.]

Eileen,

Can you be more specific?  Are you staining manually or automated?  Is your CD3 polyclonal or monoclonal?  Predilute or at what concentration?  Which HIER buffer are you using?

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: eileen dusek [mailto:eileen_dusek <@t> yahoo.com]
Sent: Friday, August 27, 2004 5:13 AM
To: histonet
Subject: [Histonet] CD3 on BM


Good Morning,
Is anyone having trouble with CD3 on BM? Our stain works great on regular tissue but weak on BM. We pressure cook the slides and yesterday I tried using an amp, still no staining. I was thinking of trying no pretreatment or protease instead of PC. Are there any other suggestions?
Sorry about the font change, I'm not sure how it happened.
I appreciate all your help
 
Eileen
Edward Hospital



		
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