[Histonet] cytology question

Richard Cartun Rcartun <@t> harthosp.org
Fri Aug 27 08:55:10 CDT 2004

It is my experience that initial fixation in alcohol can affect
immunoreactivity for those proteins that are highly soluble (e.g. S-100
and calretinin).  We also have a difficult time identifying
synaptophysin and chromogranin in alcohol-fixed cytology specimens.  We
have seen several specimens where the cytology preparations have been
negative, but tissue submitted to histology has been positive.


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> Browning Deb <browning <@t> HHSC.CA> 08/26/04 09:59AM >>>
I would like to know how many institutions make an effort to produce
blocks from FNA specimens, and whether the 50% alcohol that is used to
collect the FNA affects any IHC results.  Thanx

Debra Browning, ART
Technical Specialist, Immunohistochemistry
Anatomic Pathology
Hamilton Health Sciences
phone: (905) 527-4322  ext 46131
e-mail:  browning <@t> hhsc.ca 
fax: (905) 524-2681

This information is directed in confidence solely to the person named
and may not otherwise be distributed, copied or disclosed.  Therefore,
information should be considered strictly confidential.  If you have
received this email in error, please notify the sender immediately via
return email for further direction. Thank you for your assistance.

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 

More information about the Histonet mailing list