[Histonet] Diastase dilemma

[Joe Nocito]

Angela,
It could be that the patient section contains mucin, which will not digest
out. If this is not the case, then I suggest trying to digest the slides on
a slide rack or something to lay the slides on a horizontal surface. Just my
21/2 cents worth.

Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Angela
Bitting
Sent: Friday, August 13, 2004 2:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Diastase dilemma

Bizarre things happen here all the time, but this is REALLY BIZARRE!
We'll do a PAS w/ diastase and our control tissue will be digested but the
patient tissue won't be. They are mounted on the same slide!
The next day we will run the stain again and we tried turning the slide
upside-down in the Coplin jar. Same problem.
We mixed fresh diastase solution and tried again. Same problem.
Tomorrow, we may run it again and it will work fine.
What could be going on?
I'm perplexed!

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave.
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916


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