[Histonet] Diastase dilemma

[Angela Bitting]

Bizarre things happen here all the time, but this is REALLY BIZARRE!
We'll do a PAS w/ diastase and our control tissue will be digested but the patient tissue won't be. They are mounted on the same slide! 
The next day we will run the stain again and we tried turning the slide upside-down in the Coplin jar. Same problem.
We mixed fresh diastase solution and tried again. Same problem.
Tomorrow, we may run it again and it will work fine.
What could be going on?
I'm perplexed!

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave.
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916


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