[Histonet] Staining for Macrophages

Gayle Callis gcallis <@t> montana.edu
Thu Aug 5 09:57:05 CDT 2004

   Sorry,  but  ED-1  (CD68) is a Mouse anti-Rat antibody for a subset of
   rat  macrophages.   It may cross react to mouse but you would be doing
   doing  mouse  on  mouse IHC.     The CD68 for mouse is rat anti-mouse,
   clone FA-11 for murine peritoneal macrophages.  SEROTEC is a source of
   these antibodies, including the F4/80 (Rat anti-mouse).
   A  sidenote:   SEROTEC  has  a  free macrophage differentiation poster
   available upon request.
   At 06:58 PM 8/4/2004, you wrote:

     You can use ED-1 from Serotec.  It works on FFPE tissue with pH 6.0
     citrate antigen retrieval and on frozen tissue without AR.  You can
     also  use F4/80 on frozen tissue.  Both are rat monoclonals and you
     can  use an anti-rat secondary Ab cross adsorbed against mouse IgG.
     You  could  also  also  use ricin 1 from Vector or E-Y Laboratories
     which  binds  mouse  macrophages.   Ricin  1  is  not the dangerous
     ricin.   I  don't  know details of how to use it (I did once, but I
     don't  remember).   There  are books available or the manufacturers
     may  know  or maybe some other Histonet folks.  I have used ED1 and
     F4/80 on mouse tissues and I think it's your best bet.

     I  had  previously asked about MAC-387 staining.  Unfortunately, we
     can't  use the staining because we are staining mouse kidney and it
     doesn't  look  right  if  you  stain  with a mouse anti-body.  Does
     anyone  know  of  a  way  to  stain  for  macrophages  with  out an
     antibody???  Or perhaps a polyclonal antibody????  Thanks a bunch!
     Jennifer K. Sipes, RALAT
     Sr. Laboratory Technician
     Johns Hopkins University
     Ross 929
     720 Rutland Avenue
     Baltimore, MD  21205
     phone:  410-614-0131
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   Gayle Callis


   Research Histopathology Supervisor

   Veterinary Molecular Biology

   Montana State University - Bozeman

   PO Box 173610

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