[Histonet] Staining for Macrophages
scoop <@t> mail.nih.gov
Wed Aug 4 19:58:37 CDT 2004
You can use ED-1 from Serotec. It works on FFPE tissue with pH 6.0
citrate antigen retrieval and on frozen tissue without AR. You can
also use F4/80 on frozen tissue. Both are rat monoclonals and you
can use an anti-rat secondary Ab cross adsorbed against mouse IgG.
You could also also use ricin 1 from Vector or E-Y Laboratories which
binds mouse macrophages. Ricin 1 is not the dangerous ricin. I
don't know details of how to use it (I did once, but I don't
remember). There are books available or the manufacturers may know
or maybe some other Histonet folks. I have used ED1 and F4/80 on
mouse tissues and I think it's your best bet.
>I had previously asked about MAC-387 staining. Unfortunately, we
>can't use the staining because we are staining mouse kidney and it
>doesn't look right if you stain with a mouse anti-body. Does anyone
>know of a way to stain for macrophages with out an antibody??? Or
>perhaps a polyclonal antibody???? Thanks a bunch!
>Jennifer K. Sipes, RALAT
>Sr. Laboratory Technician
>Johns Hopkins University
>720 Rutland Avenue
>Baltimore, MD 21205
>e-mail: jengirl1014 <@t> yahoo.com
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Sharon Cooperman <scoop <@t> mail.nih.gov>
NIH, NICHD, CBMB 301.435-7735
Building 18T, room 101 301.402-0078 fax
Bethesda, MD 20892
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