[Histonet] Staining for Macrophages
Gayle Callis
gcallis <@t> montana.edu
Wed Aug 4 10:36:05 CDT 2004
There are other monoclonal antibodies for macrophages in the mouse,
generally rat antimouse, try BD Pharmingen. F4/80 (mature macrophages) is
another available from Serotec. You can use a mouse monoclonal on mouse
tissue, but should use the DAkO ARK kit or Chemicon mouse on mouse kit,
there are others on the market. Hopefully, you have a positive control
expressing the macrophage population you wish to see, this way you can
optimize your antibody before going to experimental animal.
At 08:28 AM 8/4/2004, you wrote:
>I had previously asked about MAC-387 staining. Unfortunately, we can't
>use the staining because we are staining mouse kidney and it doesn't look
>right if you stain with a mouse anti-body. Does anyone know of a way to
>stain for macrophages with out an antibody??? Or perhaps a polyclonal
>antibody???? Thanks a bunch!
>
>
>Jennifer K. Sipes, RALAT
>Sr. Laboratory Technician
>Johns Hopkins University
>Ross 929
>720 Rutland Avenue
>Baltimore, MD 21205
>phone: 410-614-0131
>cell: 443-413-0853
>e-mail: jengirl1014 <@t> yahoo.com
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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