[Histonet] cleaved caspase-3

[Jackie.O'Connor <@t> abbott.com]

I perform caspase-3 routinely using a one hour RT incubation on human 
xenografts.  I use the Pharmingen primary, and an SABC method.  Let me 
know if you'd like me to send you the protocol.  However, I am not using 
formalin fixed tissue - My tissues are zinc fixed paraffin sections.  I 
haven't been able to get consistent results with caspase-3 on ffpe.

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics
847.938.4919
Fax 847.938.3266




"Linke_Noelle" <Linke_Noelle <@t> Allergan.com>
Sent by: histonet-admin <@t> lists.utsouthwestern.edu
09/25/2003 09:37 AM

 
        To:     <histonet <@t> lists.utsouthwestern.edu>
        cc: 
        Subject:        [Histonet] cleaved caspase-3


Hi everyone!
 
Is anyone using cleaved caspase-3 with a protocol other than overnight at 
4 degrees?  I'm having problems with my staining and I don't have weeks to 
get it sorted out, so I am looking for a room temp or heated protocol to 
try.
 
Thanks in advance
 
Noelle Linke, BS, HTL(ASCP)
Allergan, Inc
Irvine, CA 92612
 

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