<br><font size=2 face="Arial">I perform caspase-3 routinely using a one hour RT incubation on human xenografts. I use the Pharmingen primary, and an SABC method. Let me know if you'd like me to send you the protocol. However, I am not using formalin fixed tissue - My tissues are zinc fixed paraffin sections. I haven't been able to get consistent results with caspase-3 on ffpe.</font>
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<br><font size=2 face="Arial">Jacqueline M. O'Connor HT(ASCP)<br>
Abbott Laboratories<br>
Global Pharmaceutical Research and Development<br>
Discovery Chemotheraputics<br>
847.938.4919<br>
Fax 847.938.3266</font>
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<td><font size=1 face="sans-serif"><b>"Linke_Noelle" <Linke_Noelle@Allergan.com></b></font>
<br><font size=1 face="sans-serif">Sent by: histonet-admin@lists.utsouthwestern.edu</font>
<p><font size=1 face="sans-serif">09/25/2003 09:37 AM</font>
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<br><font size=1 face="sans-serif"> To: <histonet@lists.utsouthwestern.edu></font>
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<br><font size=1 face="sans-serif"> Subject: [Histonet] cleaved caspase-3</font></table>
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<br><font size=2 face="Arial">Hi everyone!</font>
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<br><font size=2 face="Arial">Is anyone using cleaved caspase-3 with a protocol other than overnight at 4 degrees? I'm having problems with my staining and I don't have weeks to get it sorted out, so I am looking for a room temp or heated protocol to try.</font>
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<br><font size=2 face="Arial">Thanks in advance</font>
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<br><font size=2 face="Arial">Noelle Linke, BS, HTL(ASCP)</font>
<br><font size=2 face="Arial">Allergan, Inc</font>
<br><font size=2 face="Arial">Irvine, CA 92612</font>
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