[Histonet] isopropanol as dehydrating agent in microwave processing

[pooja chopra]

Dear Histonetter,
I am a new user of this email group. I am a resident doctor doing my thesis 
research on microwave fixation and processing in a moderately funded 
government laboratory in India. I am using a domestic microwave oven, as a 
lab oven is not affordable.
I have been able to use only isopropanol (IPA) in the microwave processing 
schedule as it is very difficult to get license for ethanol in India. I have 
read lots of discouraging things that IPA should not be used as dehydrator, 
even in microwave method. I would like to hear from someone, regarding 
whether there is any way of making use of IPA both as dehydrator and 
clearing agent.
Thanks,
Pooja Chopra

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