Hello, there is no need to dehydrate and clear after washing off of the substrate you just cover slip in an aqueous mounting medium. Best wishes. Atoska Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850