[Histonet] Fw: Brain Tissue in Buffer

[Favara, Cynthia (NIH/NIAID)]

Mary,
 
I had some investigators that insisted on putting mouse brain in PBS @4C to
insure fixation was for a uniform time. Sometimes these were in PBS[sterile]
for months. When I got them ran them through the processor with and initial
NBF station. I don't recall them being any different unless the fixation was
inadequate.
 
Good Luck!
 
c
 

Cynthia Favara 
NIAID/NIH/RML/LPVD 
903 South 4th Street 
Hamilton, MT 59840 
406-363-9317 

-----Original Message-----
From: Mary Parker [mailto:mparker <@t> epl-inc.com]
Sent: Monday, September 22, 2003 6:05 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fw: Brain Tissue in Buffer


 
----- Original Message ----- 
From: Mary Parker <mailto:mparker <@t> epl-inc.com>  
To: histonet <@t> pathology.swmed.edu.com
<mailto:histonet <@t> pathology.swmed.edu.com>  
Sent: Monday, September 22, 2003 8:03 AM
Subject: Brain Tissue in Buffer

If anyone has any experience about how to successfully process and microtome
serial sections of rat brain tissue that was perfused, then stored in buffer
solution for approx one year in the refrigerator...please help! We were
given this tissue by an outside investigator and are having a miserable time
of it.
I need all the information I can gather about tissue stored in buffer for an
extended period of time.
Is it hopeless?
Thanks
 
 
Mary Parker, H.T., A.S.C.P.
Histology Manager
EPL, Inc.
P.O. Box 12766
RTP, N.C. 27709
(919) 998-9407

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