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<DIV><SPAN class=955134712-22092003><FONT face=Georgia>Mary,</FONT></SPAN></DIV>
<DIV><SPAN class=955134712-22092003><FONT
face=Georgia></FONT></SPAN> </DIV>
<DIV><SPAN class=955134712-22092003><FONT face=Georgia>I had some investigators
that insisted on putting mouse brain in PBS @4C to insure fixation was for a
uniform time. Sometimes these were in PBS[sterile] for months. When I got them
ran them through the processor with and initial NBF station. I don't recall them
being any different unless the fixation was inadequate.</FONT></SPAN></DIV>
<DIV><SPAN class=955134712-22092003><FONT
face=Georgia></FONT></SPAN> </DIV>
<DIV><SPAN class=955134712-22092003><FONT face=Georgia>Good
Luck!</FONT></SPAN></DIV>
<DIV><SPAN class=955134712-22092003><FONT
face=Georgia></FONT></SPAN> </DIV>
<DIV><SPAN class=955134712-22092003><FONT face=Georgia>c</FONT></SPAN></DIV>
<DIV> </DIV>
<P><FONT face="BernhardFashion BT" color=#000080>Cynthia Favara</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>NIAID/NIH/RML/LPVD</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>903 South 4th Street</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>Hamilton, MT 59840</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>406-363-9317</FONT> </P>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B> Mary Parker
[mailto:mparker@epl-inc.com]<BR><B>Sent:</B> Monday, September 22, 2003 6:05
AM<BR><B>To:</B> histonet@lists.utsouthwestern.edu<BR><B>Subject:</B>
[Histonet] Fw: Brain Tissue in Buffer<BR><BR></FONT></DIV>
<DIV> </DIV>
<DIV style="FONT: 10pt arial">----- Original Message -----
<DIV style="BACKGROUND: #e4e4e4; font-color: black"><B>From:</B> <A
title=mparker@epl-inc.com href="mailto:mparker@epl-inc.com">Mary Parker</A>
</DIV>
<DIV><B>To:</B> <A title=histonet@pathology.swmed.edu.com
href="mailto:histonet@pathology.swmed.edu.com">histonet@pathology.swmed.edu.com</A>
</DIV>
<DIV><B>Sent:</B> Monday, September 22, 2003 8:03 AM</DIV>
<DIV><B>Subject:</B> Brain Tissue in Buffer</DIV></DIV>
<DIV><BR></DIV>
<DIV><FONT face=Arial size=2>If anyone has any experience about how to
successfully process and microtome serial sections of rat brain tissue that
was perfused, then stored in buffer solution for approx one year in the
refrigerator...please help! We were given this tissue by an outside
investigator and are having a miserable time of it.</FONT></DIV>
<DIV><FONT face=Arial size=2>I need all the information I can gather about
tissue stored in buffer for an extended period of time.</FONT></DIV>
<DIV><FONT face=Arial size=2>Is it hopeless?</FONT></DIV>
<DIV><FONT face=Arial size=2>Thanks</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Mary Parker, H.T., A.S.C.P.<BR>Histology
Manager<BR>EPL, Inc.<BR>P.O. Box 12766<BR>RTP, N.C. 27709<BR>(919)
998-9407</FONT></DIV></BLOCKQUOTE></BODY></HTML>