[Histonet] Opinion on storing sections on slides

[Geoff McAuliffe]

Hi Gareth:

    I would keep them in buffer in the refrigerator until I needed them. 
I have done immunos for tyrosine hydroylase and GFAP on sections stored 
at minus 80 C for several months. The GFAP was ok but the TH suffered 
from storage. Storage for just a few days at room temp ruined Mac1 for 
mouse microglia. Once sections dry out the immuno seems to go downhill.

Geoff

Davis, Gareth wrote:

>  
> Hello All,
> I would like to get some input on storing sections.  I have cut frozen 
> (PFA perfused and post fixated) tissue on a sliding microtome, kept 
> the cut sections in PBS until mounting on slides.  Then I applied the 
> sections to a SuperFrost Plus slide with water and dried down on a 
> slide warmer-overnight.  So, the staining has turned out okay, but I 
> wonder if I should keep the sections I don't use in the freezer, like 
> typical frozen sections, or keep them at room temperature, like 
> paraffin sections (since they are dry and already fixed).  I was going 
> to try to stain free floating sections, but it just didn't work.  So, 
> any suggestions would be greatly appreciated.
> Thanks,
> Gareth
>  
> Ms. Gareth B. Davis
> Research Assistant II
> Neuro-magnetics Division of
> the Department of Neurology
> Vanderbilt University Medical Center
> 615-936-3318
>  
>  


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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