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Hi Gareth:<br>
<br>
I would keep them in buffer in the refrigerator until I needed
them. I have done immunos for tyrosine hydroylase and GFAP on sections
stored at minus 80 C for several months. The GFAP was ok but the TH
suffered from storage. Storage for just a few days at room temp ruined
Mac1 for mouse microglia. Once sections dry out the immuno seems to go
downhill.<br>
<br>
Geoff<br>
<br>
Davis, Gareth wrote:<br>
<blockquote type="cite"
cite="mid082C721AF78DB34983E8BA2CD085462105C676@mailbe07">
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<div>Hello All,</div>
<div>I would like to get some input on storing sections. I have cut
frozen (PFA perfused and post fixated) tissue on a sliding microtome,
kept the cut sections in PBS until mounting on slides. Then I applied
the sections to a SuperFrost Plus slide with water and dried down on a
slide warmer-overnight. So, the staining has turned out okay, but I
wonder if I should keep the sections I don't use in the freezer, like
typical frozen sections, or keep them at room temperature, like
paraffin sections (since they are dry and already fixed). I was going
to try to stain free floating sections, but it just didn't work. So,
any suggestions would be greatly appreciated.</div>
<div>Thanks,</div>
<div>Gareth</div>
<div> </div>
<div><font face="System" color="#800080" size="2">Ms. Gareth B. Davis</font></div>
<div><font face="System" color="#800080" size="2"><strong>Research
Assistant II</strong></font></div>
<div><font face="System" color="#800080" size="2">Neuro-magnetics
Division of </font></div>
<div><font face="System" color="#800080" size="2">the Department of
Neurology</font></div>
<div><font face="System" color="#800080" size="2">Vanderbilt
University Medical Center</font></div>
<div><font face="System" color="#800080" size="2">615-936-3318</font></div>
<div> </div>
<div> </div>
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<br>
<pre class="moz-signature" cols="72">--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
<a class="moz-txt-link-abbreviated" href="mailto:mcauliff@umdnj.edu">mcauliff@umdnj.edu</a>
**********************************************</pre>
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