[Histonet] Opinion on storing sections on slides

[Davis, Gareth]

Hello All,
I would like to get some input on storing sections.  I have cut frozen (PFA perfused and post fixated) tissue on a sliding microtome, kept the cut sections in PBS until mounting on slides.  Then I applied the sections to a SuperFrost Plus slide with water and dried down on a slide warmer-overnight.  So, the staining has turned out okay, but I wonder if I should keep the sections I don't use in the freezer, like typical frozen sections, or keep them at room temperature, like paraffin sections (since they are dry and already fixed).  I was going to try to stain free floating sections, but it just didn't work.  So, any suggestions would be greatly appreciated.
Thanks,
Gareth
 
Ms. Gareth B. Davis
Research Assistant II
Neuro-magnetics Division of 
the Department of Neurology
Vanderbilt University Medical Center
615-936-3318
 
 
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