[Histonet] Purpose of Oven Prior to Staining?

[Kemlo Rogerson]

How could the oven de-wax the section? The wax is in the tissue isn't
it? Does it drain out? Nah, xylene removes the wax.

The oven gets rid of the water, allows the section to fully 'uncrinkle'
and brings the molecules of the tissue closer to that of the glass, thus
causing intermolecular forces to keep the section on the slide (my
hypothesis). I mean how else do sections adhere to glass if the slide is
not treated?

How do the sections stay on the glass? Any ideas? 

Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS
Tel: 01270 877625
Mob: 07830 196072 
Mobile E-Mail kemlorogerson <@t> 3mail.com                     
FAX & Answer Phone 0871 242 8094
E-mail Accounts:  
             kemlo <@t> tiscali.co.uk or 
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-----Original Message-----
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu] On Behalf Of Jordan
Brod
Sent: 18 September 2003 15:41
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Purpose of Oven Prior to Staining?

Good Morning All:

My lab is in a huge discussion about the reason why we put our slides in
an oven before staining.  I told them that the reason was to dry all of
the water off of them, because xylene and water do not mix.  This
statement is also backed up in literature that I have read.  They still
do not believe me, because they think the purpose of the oven is to
deparaffinize the slides.

Can anyone help me out, so I can put this discussion to rest?  I will
print off all of your comments for my technicians to read.  Is what I
told them correct or not?

Thanks.


 

********************************************************************
Jordan W. Brod
Diagnostic Lab Supervisor - Pathology
Texas Veterinary Medical Diagnostic Laboratory (TVMDL)
1 Sippel Road
TAMU 4471
College Station, Texas 77843
(979) 845-3414
(979) 845-1794 fax
jbrod <@t> tvmdl.tamu.edu


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