[Histonet] Purpose of Oven Prior to Staining?

[Geoff McAuliffe]

Hi Jordan:

    I'm with you on this one. If the oven will deparafinize sections it 
should be possible to stain sections with an aqueous stain after 
"deparafinization" in the oven. Have them try this while you remove wax 
the normal way. Compare results. Maybe then they will believe you 
(unelss they are very stubborn).

Geoff

Jordan Brod wrote:

>Good Morning All:
>
>My lab is in a huge discussion about the reason why we put our slides in an oven before staining.  I told them that the reason was to dry all of the water off of them, because xylene and water do not mix.  This statement is also backed up in literature that I have read.  They still do not believe me, because they think the purpose of the oven is to deparaffinize the slides.
>
>Can anyone help me out, so I can put this discussion to rest?  I will print off all of your comments for my technicians to read.  Is what I told them correct or not?
>
>Thanks.
>
>
> 
>
>********************************************************************
>Jordan W. Brod
>Diagnostic Lab Supervisor - Pathology
>Texas Veterinary Medical Diagnostic Laboratory (TVMDL)
>1 Sippel Road
>TAMU 4471
>College Station, Texas 77843
>(979) 845-3414
>(979) 845-1794 fax
>jbrod <@t> tvmdl.tamu.edu
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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