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<DIV>Hello All,</DIV>
<DIV>I would like to get some input on storing sections. I have cut frozen
(PFA perfused and post fixated) tissue on a sliding microtome, kept the cut
sections in PBS until mounting on slides. Then I applied the sections to a
SuperFrost Plus slide with water and dried down on a slide
warmer-overnight. So, the staining has turned out okay, but I wonder if I
should keep the sections I don't use in the freezer, like typical frozen
sections, or keep them at room temperature, like paraffin sections (since they
are dry and already fixed). I was going to try to stain free floating
sections, but it just didn't work. So, any suggestions would be greatly
appreciated.</DIV>
<DIV>Thanks,</DIV>
<DIV>Gareth</DIV>
<DIV> </DIV>
<DIV><FONT face=System color=#800080 size=2>Ms. Gareth B. Davis</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2><STRONG>Research Assistant
II</STRONG></FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>Neuro-magnetics Division of
</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>the Department of
Neurology</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>Vanderbilt University Medical
Center</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>615-936-3318</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2></FONT> </DIV>
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