[Histonet] Purpose of Oven Prior to Staining?

[Jordan Brod]

Good Morning All:

My lab is in a huge discussion about the reason why we put our slides in an oven before staining.  I told them that the reason was to dry all of the water off of them, because xylene and water do not mix.  This statement is also backed up in literature that I have read.  They still do not believe me, because they think the purpose of the oven is to deparaffinize the slides.

Can anyone help me out, so I can put this discussion to rest?  I will print off all of your comments for my technicians to read.  Is what I told them correct or not?

Thanks.


 

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Jordan W. Brod
Diagnostic Lab Supervisor - Pathology
Texas Veterinary Medical Diagnostic Laboratory (TVMDL)
1 Sippel Road
TAMU 4471
College Station, Texas 77843
(979) 845-3414
(979) 845-1794 fax
jbrod <@t> tvmdl.tamu.edu