[Histonet] Bouin's fixation/removal of picric acid

[John Kiernan]

Sarah, do you use the saturated lithium 
carbonate in 70% ethanol on blocks of
tissue or on hydrated paraffin sections
that are yellow?  

If it's for blocks, how do you remove
lithium carbonate from the decolorized
specimens?  
              John Kiernan
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan <@t> uwo.ca
   http://publish.uwo.ca/~jkiernan/
___________
Sarah Jones wrote:
> 
> I've always used saturated lithium carbonate in 70% ethanol to remove
> the picric acid.  I believe it would be faster and more complete than
> 70% alone.
> 
> Sarah Jones HT(ASCP)
> Dept. of Vet. Anatomy & Public Health
> Histology Lab
> Texas A&M University
> College Station, TX 77843-4458
> phone: 979-845-3177
> fax:  979-458-3499
> 
> >>> "peptolab" <peptolab <@t> hamptons.com> 09/13/03 08:32AM >>>
> A 3-5 mm testicular biopsy should be fixed in one to two hours. Be sure
> to
> rinse out as much yellow picric acid as possible (you can use running
> water
> or 70% alcohol ). The basophilia of chromatin in the stored block will
> deteriorate after some time if there is picric acid  residue.
> 
> Jeff Silverman HT HTL QIHC (ASCP)
> Southside Hospital
> Bay Shore NY
>